文献类型：期刊文献

英文题名：A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3)

作者：Zhao, Xi-Hua[1,2];Wang, Wei[1];Wang, Feng-Qing[1];Wei, Dong-Zhi[1]

机构：[1]E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China

年份：2012

卷号：110

起止页码：539

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305852700077)】；

基金：This work was supported by National Basic Research Program of China (973) Program No. 2009CB724703.

语种：英文

外文关键词：beta-1, 4-Endoglucanase; Escherichia coli Rosetta (DE3); Pichia pastoris GS115; Glycosylation; Biostoning

摘要：beta-1, 4-Endoglucanase (EG) from Bacillus subtilis LH was expressed in Escherichia coli Rosetta (DE3) and Pichia pastoris GS115, respectively. The CMCase activity of EG (EGE) from the cell lysate of DE3 reached 20,010 U/ml, and that of EG (EGP) from the supernatant of GS115 was only 2008 U/ml. EGE and EGP were bifunctional cellulases excluding beta-1, 4-glucosidase (BGL). The CMCases of them, optimally active at 65 degrees C and pH 6.8, exhibited more than 80% residual activity at pH 5-10 and 60% activity at 40-70 degrees C and pH 5-9. EGE (EGP) mixed with BGL had more than 1.5-fold higher CMCase and filter paperase activities compared to EGE (EGP). N-glycosylation protected EGP from immobilized-papain attack and accounted for 30 kDa and a higher thermostability, whereas EGE was decomposed into a 33 kDa active truncated EG (EGT) and two 18 kDa fragments. EGE and EGP performed much better than EGT in denim biostoning. (C) 2011 Elsevier Ltd. All rights reserved.