文献类型：期刊文献

英文题名：Preparation and evaluation of open-tubular capillary columns modified with metal-organic framework incorporated polymeric porous layer for liquid chromatography

作者：Zhu, Manman[1];Zhang, Lingyi[1];Chu, Zhanying[1];Wang, Shulei[1];Chen, Kai[1];Zhang, Weibing[1];Liu, Fan[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai, Peoples R China;[2]Nanchang Univ, Ctr Anal & Testing, Nanchang, Jiangxi, Peoples R China

年份：2018

卷号：184

起止页码：29

外文期刊名：TALANTA

收录：;EI(收录号：20181004860182);WOS:【SCI-EXPANDED(收录号:WOS:000432234900005)】；

基金：We gratefully acknowledged the support of the National Natural Science Foundation of China (No. 21475044), National Key Scientific Instrument and Equipment Development Project (2012YQ120044) and the National Key Technology R&D Program (2015BAK44B00).

语种：英文

外文关键词：Metal organic framework; Open-tubular capillary column; Liquid chromatography

摘要：An open tubular capillary liquid phase chromatographic column (1 m x 25 mu m i.d. x 375 mu m o.d.) was prepared by incorporating metal organic framework particles modified with vancomycin directly into zwitterionic polymer coating synthesized by the copolymerization of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide and N,N'-methylenebisacrylamide. The incorporation of IRMOF-3 (isoreticular metal organic framework-3) particles improved selectivity of zwitterionic polymer coating with absolute column efficiency reaching 79900 plates for p-xylene. Besides strong hydrophilic interaction, the separation of neutral, basic, and acidic compounds demonstrates that pi-pi stacking interaction and the coordination effect of unsaturated Zn2+ of MOF also contribute to separation of various analytes. The RSD values (run-to-run, day-to-day, column-to-column, n = 3) of retention time of neutral compounds were less than 0.71%, 0.69% and 3.08% respectively, suggesting good repeatability. In addition, the column was applied to the analysis of the trypsin digest of bovine serum albumin, revealing the potential in separating biological samples.