文献类型：期刊文献

英文题名：Photoacoustic probes for real-time tracking of endogenous H2S in living mice

作者：Shi, Ben[1];Gu, Xianfeng[2];Fei, Qiang[1];Zhao, Chunchang[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Fudan Univ, Sch Pharm, Dept Med Chem, Shanghai 201203, Peoples R China

年份：2017

卷号：8

期号：3

起止页码：2150

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20171003408570);WOS:【SCI-EXPANDED(收录号:WOS:000395906900057)】；

基金：We gratefully acknowledge the financial support from the National Science Foundation of China (grant numbers 21172071, 21190033, 21372083, 21572039), NSFC for Creative Research Groups (21421004), National 973 Program (2013CB733700) and the Programme of Introducing Talents of Discipline to Universities (B16017). We thank Prof. Ping Shi and Yajing Liu for assistance with in vivo imaging.

语种：英文

外文关键词：Biocompatibility - Photoacoustic effect - Tumors - Mammals - Infrared devices

摘要：H2S is a key chemical mediator that exerts a vital role in diverse physiological and pathological processes. However, in vivo tracking of endogenous H2S generation still remains difficult due to the lack of reliable analytical methods. Herein, we present the first example of activatable photoacoustic probes for real-time imaging of H2S in living mice through the full utilization of the superiority of photoacoustic imaging modality at fine spatial resolution during deep tissue penetration. The designed probe can generate high NIR absorption at 780 nm in the presence of H2S, thus producing a strong photoacoustic signal output in the NIR region. Furthermore, this probe exhibits extremely fast and highly selective responsiveness, good water-solubility and excellent biocompatibility. In light of these outstanding features, this probe realizes the direct photoacoustic trapping of endogenous H2S generation in a HCT116 tumor-bearing mouse model. These preliminary imaging studies show that HCT116 colon tumors exhibit CBS upregulation activity, resulting in an increased rate of H2S generation.