文献类型：期刊文献

英文题名：Rational construction of probes rendering ratiometric response to the cancer-specific enzyme NQO1

作者：Fei, Qiang[1,2,3];Zhou, Li[4];Wang, Feiyi[2,3];Shi, Ben[2,3];Li, Chunbao[1];Wang, Rui[4];Zhao, Chunchang[2,3]

机构：[1]Tianjin Univ, Sch Sci, Dept Chem, Tianjin 300072, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China

年份：2017

卷号：136

起止页码：846

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20164002866438);WOS:【SCI-EXPANDED(收录号:WOS:000387837200101)】；

基金：We gratefully acknowledge the financial support by the National Science Foundation of China (grant numbers 21172071, 21190033, 21372083), National 973 Program (2013CB733700) and the Programme of Introducing Talents of Discipline to Universities (B16017).

语种：英文

外文关键词：Cancer biomarker; Polymeric micelle; Nanoprobe; Ratiometric; Cancer-specific NQO1

摘要：Pursuit of fluorescent probes for identification of cancer biomarkers benefits reliable predictions of early cancer detection. Here, a strategy employing polymeric micelle assembly for encapsulation of a new enzyme (NQO1)-responsive small molecule was established for facile access to nanoprobes with favorable features in bioimaging, such as good aqueous solubility, enhanced photo-stability and biocompatibility. In the presence of the enzyme NQO1, these nanoprobes underwent ratiometric fluorescence changes from yellow-green fluorescence signals to red fluorescence signals. By exploiting this favorable ratiometric response to NQO1, we explored these nanoprobes for evaluation of enzyme activity in living cells, which showed distinct differentiation between NQO1-positive cancer cells and NQO1-negative cells. (C) 2016 Elsevier Ltd. All rights reserved.