文献类型：期刊文献

中文题名：微波法提取紫苏黄酮类物质及其成分分析

英文题名：Microwave-Assisted Extraction and Composition Analysis of Flavonoids from Perilla frutescens Leaves

作者：张蕾蕾[1];常雅宁[1];夏鹏竣[1];俞建瑛[1];徐炎鹏[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：2012

卷号：33

期号：22

起止页码：53

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：上海市科委重大应用专项(04-027;07-122;09-121)

语种：中文

中文关键词：微波;紫苏;黄酮类物质;响应面优化;柱层析;全波长扫描;高效液相色谱法

外文关键词：microwave; Perilla frutescens; flavonoids; response surface methodology; column chromatography; full wavelength scan; high performance liquid chromatography（HPLC）

摘要：研究微波法提取紫苏黄酮类物质的最佳工艺条件。采用单因素及响应面优化试验,分别考察紫苏粒径、提取时间、微波温度、乙醇体积分数及料液比对提取率的影响。结果表明:紫苏粒径20目、提取时间70min、温度71℃、乙醇体积分数61%、料液比1:20(g/mL)时,黄酮得率最高为59.28mg/g。紫苏提取液经柱层析法纯化后,经全波长扫描和高效液相色谱显示,该物质主要成分为芦丁、木犀草素等。
Response surface methodology was used to optimize conditions for the microwave-assisted extraction of flavonoids from Perilla frutescens leaves.The effects of raw material particle size,extraction time,temperature,ethanol concentration and solid-to-solvent ratio on extraction efficiency were investigated.The optimal conditions for extracting flavonoids from Perilla frutescens leaves were found to be extraction at 71 ℃ for 70 min with 61% ethanol at a solid-to-solvent ratio of 1：20（g/mL） and a raw material particle size of 20 mesh.Under these conditions,the maximum extraction yield of flavonoids was 59.28 mg/g.The obtained extract was purified by D152 macroporous resin column chromatography and the purified product mainly consisted of lutin and luteolin as demonstrated by UV absorption spectrometry and HPLC.