文献类型：期刊文献

英文题名：Ginsenoside Rd-Loaded Antioxidant Polymersomes to Regulate Mitochondrial Homeostasis for Bone Defect Healing in Periodontitis

作者：Hu, Congjiao[1,2,3];Shi, Junqiu[4,5];Zhang, Fan[1,2,3];Lv, Mingchen[5];Ge, Zhenghong[5];Feng, Meiting[1,2,3];Fan, Zhen[4,5];Liu, Danqing[6];Du, Jianzhong[4,5,6,7];Sun, Yao[1,2,3]

机构：[1]Tongji Univ, Shanghai Tongji Stomatol Hosp & Dent Sch, Shanghai Engn Res Ctr Tooth Restorat & Regenerat, Shanghai 200072, Peoples R China;[2]Tongji Univ, Tongji Res Inst Stomatol, Shanghai 200072, Peoples R China;[3]Shanghai Tongji Stomatol Hosp & Dent Sch, Dept Implantol, Shanghai 200072, Peoples R China;[4]Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Sch Med,Dept Gynaecol & Obstet,Shanghai Key Lab An, Shanghai 200434, Peoples R China;[5]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China;[6]Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Orthoped, Shanghai 200072, Peoples R China;[7]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：14

期号：4

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20245217571123);WOS:【SCI-EXPANDED(收录号:WOS:001380222800001)】；

基金：C.H. and J.S. contributed equally to this work. This work was financially supported by National Key R&D Program of China (2022YFA1103200, 2022YFC2402900), National Natural Science Foundation of China (81822012, 82061130222, 22305176, 81771043, 82270963, U23A20444, 82425014, 21925505, 22335005, 22075212), Shanghai Experimental Animal Research Project of Science and Technology Innovation Action Plan (8191101676), Shanghai Academic Leader of Science and Technology Innovation Action Plan (20XD1424000), Innovation Program of Shanghai Municipal Education Commission (2023ZKZD28, 2023ZKZD29), Shanghai International Scientific Collaboration Fund (21520710100), and the China Postdoctoral Science Foundation (2024T170667, 2023M732630).

语种：英文

外文关键词：mitochondrial homeostasis; mitophagic flux; periodontitis; ROS-responsive polymersomes; self-assembly

摘要：Periodontitis is the leading cause of tooth loss in adults. Initially triggered by bacterial infection, it is characterized by subsequent dysregulation of mitochondrial homeostasis, leading to ongoing loss of periodontal tissue. Mitophagic flux, a critical physiological mechanism for maintaining mitochondrial homeostasis, is compromised in periodontitis. Additionally, increased release of reactive oxygen species (ROS) exacerbates mitochondrial damage. In this study, a ginsenoside Rd (Rd)-loaded antioxidative polymersome (RdAP) is designed, which is self-assembled from a mitochondrial-protective and ROS-scavenging block copolymer, poly(ethylene oxide)-block-poly(phenylboronic acid pinacol ester-conjugated polylysine) (PEO113-b-P(Lys-PAPE)60). The phenylboronic acid pinacol ester (PAPE) segment exhibits excellent ROS-responsive properties, enabling effective ROS scavenging through antioxidant production. Rd significantly enhances mitophagic flux by 2.5-fold in periodontal ligament stem cells (PDLSCs) under oxidative stress. Together with the antioxidative polymersome, RdAPs restore mitochondrial homeostasis and enhance the osteogenic capacity of PDLSCs, bringing it closer to that of healthy controls. In a mouse model of periodontitis, the bone mass in the RdAP-treated group is 1.37 times greater than that in the untreated periodontitis group. Overall, the findings propose a novel strategy for addressing refractory periodontitis, which may also be applicable to other diseases characterized by mitochondrial homeostasis imbalance.