文献类型：期刊文献

英文题名：Efficient production of succinic acid in engineered Escherichia coli strains controlled by anaerobically-induced nirB promoter using sweet potato waste hydrolysate

作者：Huang, Meihua[1,2];Cheng, Jie[1,2];Chen, Peng[1];Zheng, Gaowei[2];Wang, Dan[1,2];Hu, Yuanliang[3]

机构：[1]Chongqing Univ, Dept Chem Engn, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Hubei Normal Univ, Coll Life Sci, Hubei Key Lab Edible Wild Plants Conservat & Util, Huangshi 435002, Hubei, Peoples R China

年份：2019

卷号：237

起止页码：147

外文期刊名：JOURNAL OF ENVIRONMENTAL MANAGEMENT

收录：;EI(收录号：20233414609640);WOS:【SCI-EXPANDED(收录号:WOS:000465059900017)】；

基金：We would like to thank the financial support from the National Natural Science Foundation of China (31501468), the Fundamental Research Funds for the Central Universities (Project No. 106112017CDJXFLX0014, 2018CDQYHG0010), the Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, the grant EWPL201507 of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization. This work also is partially supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, Shanghai, China.

语种：英文

外文关键词：Anaerobically-induced nirB promoter; Sweet potato waste; Succinic acid; Engineered Escherichia coil

摘要：Succinic acid has attracted interest worldwide as a precursor of many industrially crucial chemicals. Biosynthesis of succinic acid from biomass is developing as an environmentally friendly strategy now. Conversion of sweet potato waste (SPW) to succinic acid could implement high-value utilization of biomass, cut cost of the fermentation process and reduce the pollution of environment. Engineered Escherichia colt (E. coli) strain HD134 under the control of anaerobically-induced nirB promoter from Salmonella enterica (P-snirB) could produce about 16.30 g/L succinic acid with a yield of 0.83 g/g after 48 h on glucose. With SPW hydrolysate as the substrate, 18.65 g/L succinic acid with a yield of 0.94 g/g after 48 h fermentation achieved. Compared to SD134 under Trc control induced with Isopropyl beta-D-Thiogalactoside (IPTG), this concentration and yield represented an 8.56% and 6.82% increase, respectively. The use of anaerobically-induced P-snirB not only could attain higher production of succinic acid than IPTG-induced Trc promoter, but omit cost of expensive exogenous inducers. The efficient production of succinic acid from SPW was firstly studied by anaerobically-induced P-snirB control, which achieved relative lower cost compared to glucose as substrate and IPTG as the inducer. This novel fermentation process conduces to the cosmically industrial succinic acid bioproduction.