文献类型：期刊文献

中文题名：MTT法测定人表皮癌细胞的抗药性

英文题名：Determination of Multidrug Resistance of Human KB Carcinoma Cells by MTT Colorimetric Assay

作者：钱江潮[1];魏东芝[1];陈笑岚[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所上海200237

年份：2000

卷号：26

期号：1

起止页码：103

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金!资助项目(39680032);国家科委医药技术创新博士项目

语种：中文

中文关键词：MTT法;多药抗性;肿瘤;化疗药物

外文关键词：MTT; colorimetric assay; serum; cell viability; chemotherapeutical drug

摘要：在用ＭＴＴ测定细胞存活率以研究人表皮癌细胞ＫＢ－３－１ 及其抗阿霉素细胞株ＫＢ－Ａ－１对化疗药物阿霉素、长春花碱和秋水仙碱的多药抗性时，发现培养基中血清浓度的变化对ＭＴＴ法测定结果有影响。吸光值在血清浓度为ｗ ＝ ０．０２ 时最高，ｗ ＝ ０．３０ 时最低，ｗ ＝ ０．０５～０．２０ 之间波动不大。而不加血清使甲酉尤溶解困难而产生误差。培养基中的血清（常为ｗ ＝ ０．１０）不影响ＭＴＴ法的结果，在完全培养基中用ＭＴＴ法测定了两株细胞的抗药性。ＫＢ－Ａ－１ 对阿霉素、长春花碱和秋水仙碱的相对抗药性分别为ＫＢ－３－１ 的１７６、４５ 和１３７ 倍。ＭＴＴ法可简便地用于抗癌药物的筛选。
To determine the relative resistances of KB A 1 cells to the parent cell line KB 3 1,MTT colorimetric assay was used to evaluate the cell growth and survival.In the experiment,It was found that the serum can affect the result of MTT assay,and the optical density reached the maximum with the serum concentration of w =0.02,and the minimum with w =0.30.While at the concentrations between w =0.05~0.20 it fluctuated a little.When no serum was added,the formazan product could not be easily dissolved and the error was inevitably introduced.So,in the ordinary range around w =0.10,the addition of serum to the reaction medium will not cause error to the MTT assay and the experiment can be done easily.This MTT assay was used to perform drug sensitivity assays of KB 3 1 and its drug resistant mutant,KB A 1.The relative resistances of KB A 1 to colchicine,vinblastine and adriamycin are 137,45 and 167 respectively compared with KB 3 1.