文献类型：期刊文献

英文题名：De Novo Multienzyme Synthetic Pathways for Lactic Acid Production

作者：Ding, Xu-Wei[1];Rong, Jing[1];Pan, Ze-Peng[1];Zhu, Xin-Xin[1];Zhu, Zhen-Yu[1];Chen, Qi[1];Zhang, Zhi-Jun[1];Xu, Jian-He[1];Li, Chun-Xiu[1];Zheng, Gao-Wei[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：14

期号：7

起止页码：4665

外文期刊名：ACS CATALYSIS

收录：;EI(收录号：20241215757273);WOS:【SCI-EXPANDED(收录号:WOS:001184793500001)】；

基金：This work was supported by the National Key Research and Development Program of China (No. 2022YFC2105900) and the Shanghai Pilot Program for Basic Research (No. 22TQ1400100-2).

语种：英文

外文关键词：biocatalysis; C1 compound; lacticacid; multienzyme cascade; protein engineering

摘要：The utilization and conversion of one-carbon (C1) feedstocks through biological processes have attracted much interest in building a carbon cycle economy. However, relevant research on the biosynthetic pathway of lactic acid (LA) from C1 compounds has not been reported. Herein, three de novo multienzyme synthetic pathways for optically pure and racemic LA from methanol were successfully engineered using a modularized construction and optimization approach involving enzyme screening, directed evolution, and improving reaction conditions. The general synthetic system achieved a productivity of 2.2-2.8 g/L LA with a maximum synthesis rate ranging from 9.6 to 15.6 g/L/day. Both L- and D-LA showed high stereoselectivity (>99% ee). This work provides an eco-friendly alternative approach for the production of LA, and it could contribute to carbon neutrality in the future.