文献类型：期刊文献

英文题名：Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401

作者：He, Yu-Cai[1,2];Xu, Jian-He[1];Su, Jin-Huan[1];Zhou, Li[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266071, Peoples R China

年份：2010

卷号：160

期号：5

起止页码：1428

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20101112767948);WOS:【SCI-EXPANDED(收录号:WOS:000274398500016)】；

语种：英文

外文关键词：Nitrilase; Alcaligenes sp; ECU0401; Glycolic acid; Glycolonitrile; Immobilization

摘要：Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and Cu2+, respectively. Glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mM glycolonitrile in the mode of sequential addition during the cultivation of Alcaligenes sp. ECU0401 in a 5-L jar fermenter. Fifty micromolars of glycolonitrile could be hydrolyzed in a yield of 94.1% by resting cells after 36 h. The microbial nitrilase system could hydrolyze various nitriles with high activity, and no amidase activity and glycolic acid were observed in hydrolyzing glycolamide. It significantly exhibited high enantioselectivity in the hydrolysis of mandelonitrile and 2-chloromandelonitrile (> 99.9% e.e. (p) ). Efficient biocatalyst recycling was achieved as a result of immobilization in glutaraldehyde/polyethylenimine cross-linked carrageenan with immobilized cells exhibiting a biocatalyst productivity of 1,042.2 g glycolic acid per gram dry cell weight after 29 batch recycles.