文献类型：期刊文献

英文题名：Self-folded three-dimensional RNA origami: Directly sprayable RNAi biopesticide for lepidopteran pest control

作者：Pang, Bailu[1];Han, Hongyu[1];Zhang, Jiaxin[1];Tan, Yongan[2];Jiang, Yiping[2];Xu, Zhiping[1];Xu, Yufang[1];Zhu, Weiping[1];Yang, Yangyang[1];Qian, Xuhong[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Inst Plant Protect, Jiangsu Acad Agr Sci, 50 Zhongling St, Nanjing 210014, Peoples R China

年份：2026

卷号：536

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20260014498);WOS:【SCI-EXPANDED(收录号:WOS:001741184000001)】；

基金：This work is supported by Shanghai Agricultural Science and Tech-nology Innovation Project (T20252013 and K2025007) and National Key Research and Development Program of China (2023YFD1700300 and 2022YFD1700400) .

语种：英文

外文关键词：RNA origami; RNA biopesticide; Spraying induced gene silencing; Lepidopteran pest; Sustainable agriculture

摘要：RNA interference (RNAi)-based biopesticides have emerged as a revolutionary tool for sustainable agriculture. However, their efficacy is hampered by the instability of RNAs and multilayered barriers in pests, and particularly RNAi biopesticides are most unsusceptible to lepidopterans pest. Although the use of nanocarriers has enhanced their stability and RNAi efficiency, some inherent drawbacks remain, such as formulation complexity and the uncertainty of nanocarriers. Herein, we utilize the single-stranded RNA self-assembly technique to construct three-dimensional (3D) tetrahedral-shaped RNA origami, an RNA biopesticide with potential for sprayable formulation,termed T-RiOBs. The T-RiOBs display superior foliar wettability, efficient absorption in plants. Notably, excellent penetration performances of T-RiOB are observed in the midguts and body walls of the larvae of Aedes albopictus and lepidopteran pest, Spodoptera exigua (S. exigua), indicating T-RiOB not only exhibits enhanced stability in the intestine but promotes body accumulation. These findings strongly suggest that T-RiOB shows potential for field spraying applications. Most importantly, without the assistance of any additional carrier, T-RiOB demonstrates control potential against S. exigua, by effectively silencing the vacuolar-type ATPase (VATPase) gene, significantly inhibiting larval growth, and markedly increasing mortality. This technique presents a universal, and applicable platform for advancing RNA biopesticides in crop protection.