文献类型：期刊文献

英文题名：Lighting up arginine metabolism reveals itsfunctional diversity in physiology and pathology

作者：Li, Rui[1,2];Li, Yan[1,3];Jiang, Kun[1,2];Zhang, Lijuan[1];Li, Ting[1,2];Zhao, Aihua[4];Zhang, Zhuo[1,2];Xia, Yale[1];Ge, Kun[4];Chen, Yaqiong[1];Wang, Chengnuo[1];Tang, Weitao[1];Liu, Shuning[1];Lin, Xiaoxi[5];Song, Yuqin[6];Mei, Jie[1];Xiao, Chun[1];Wang, Aoxue[1,2];Zou, Yejun[1,2];Li, Xie[1,2];Chen, Xianjun[1,2];Ju, Zhenyu[7];Jia, Wei[4];Loscalzo, Joseph[8];Sun, Yu[3];Fang, Wei[5];Yang, Yi[1];Zhao, Yuzheng[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Shanghai Frontiers Sci Ctr Optogenet Tech CellMeta, Sch Pharm,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Med Sci, Res Unit New Tech L Cell Metab Imaging, Beijing 100730, Peoples R China;[3]Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Chinese Acad Sci, Key Lab Tissue Microenvironm & Tumor, Shanghai 200031, Peoples R China;[4]Shanghai Jiao Tong Univ, Ctr Translat Med, Affiliated Peoples Hosp 6, Shanghai Key Lab Diabet Mellitus, Shanghai 200233, Peoples R China;[5]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Laser & Aesthet Med, Sch Med, Shanghai 200011, Peoples R China;[6]Suzhou Ruijin Vitiligo Med Res Inst, Suzhou 215100, Peoples R China;[7]Jinan Univ, Inst Aging & Regenerat Med, Key Lab Regenerat Med, Guangzhou Regenerat Med & Hlth Guangdong Lab,Minis, Guangzhou 510632, Peoples R China;[8]Brigham & Womens Hosp, Harvard Med Sch, Dept Med, Boston, MA 02115 USA

年份：2025

卷号：37

期号：1

起止页码：291

外文期刊名：CELL METABOLISM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001401247600001)】；

基金：We thank Stephanie C. Tribuna for secretarial assistance. We thank Jiaoni Chi, Nan Liang, Hang Dai, Yuxin Cheng, and Zhuohang Yu for technical assistance. This research is supported by the National Key Research and Development Program of China (2019YFA0904800 to Y. Zhao, 2022YFC3400100 to Y.Y., and 2023YFA1802000 to T.L.) , NSFC (92357308, 32150030, 32030065, 32121005, and 92049304 to Y. Zhao and Y.Y., 82030039 to Z.J., and 31901033 to T.L.) , and the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Y. Zhao) . Research Unit of New Techniques for Live-cell Metabolic Imaging (Chinese Academy of Medical Sciences, 2019-I2M-5-013 to Y. Zhao) , Postdoctoral Fellowship Program of CPSF (GZC20230805 to K.J.) , Innovative research team of high-level local universities in Shanghai, the State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities, Research Physician Training Project from Shanghai Ninth People's Hospital, US National Institutes of Health (HL155107, HL155096, HL166137, and HG007690 to J.L.) , and the American Heart Association (D700382 and AHA2020CV-19 to J.L.) .

语种：英文

摘要：Arginine is one of the most metabolically versatile amino acids and plays pivotal roles in diverse biologicaland pathological processes; however, sensitive tracking of arginine dynamicsin situremains technicallychallenging. Here, we engineer high-performance fluorescent biosensors, denoted sensitive to arginine(STAR), to illuminate arginine metabolism in cells, mice, and clinical samples. Utilizing STAR, we demonstratethe effects of different amino acids in regulating intra- and extracellular arginine levels. STAR enabled live-cellmonitoring of arginine fluctuations during macrophage activation, phagocytosis, efferocytosis, and senes-cence and revealed cellular senescence depending on arginine availability. Moreover, a simple and fast assaybased on STAR revealed that serum arginine levels tended to increase with age, and the elevated serum argi-nine level is a potential indicator for discriminating the progression and severity of vitiligo. Collectively, ourstudy provides important insights into the metabolic and functional roles of arginine, as well as its potentialin diagnostic and therapeutic applications.