文献类型：期刊文献

英文题名：Pre-treatment of rats with ad-hepcidin prevents iron-induced oxidative stress in the brain

作者：Gong, Jing[1,3];Du, Fang[2,3];Qian, Zhong Ming[1,3];Luo, Qian Qian[3];Sheng, Yuan[3];Yung, Wing-ho[2];Xu, Yan Xin[1];Ke, Ya[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Shanghai 200237, Peoples R China;[2]Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China;[3]Fudan Univ, Neuropharmacol Lab, Sch Pharm, Shanghai 201203, Peoples R China

年份：2016

卷号：90

起止页码：126

外文期刊名：FREE RADICAL BIOLOGY AND MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000367396600012)】；

基金：The studies in our laboratories were supported by the Competitive Earmarked Grants of The Hong Kong Research Grants Council (GRF 466713) Hong Kong Health and Medical Research Fund (01120146), National 973 Programs (2011CB510004), the General Grant of National Natural Science Foundation of China (NSFC) (31271132 and 31371092), and Key Project Grant of NSFC (31330035).

语种：英文

外文关键词：Hepcidin; brain iron; Reactive oxygen species (ROS); Cortex; Hippocampus; Striatum; Substantia nigra; Iron-overloaded rat

摘要：Our recent investigation showed that hepcidin can reduce iron in the brain of iron-overloaded rat by down-regulating iron-transport proteins. It has also been demonstrated that iron is a major generator of reactive oxygen species. We therefore hypothesized that hepcidin could prevent iron accumulation and thus reduce iron-mediated oxidative stress in iron-overloaded rats. To test this hypothesis, we investigated the effects of pre-treatment of rats with recombinant-hepcidin-adenovirus (ad-hepcidin) on the contents of iron, dichlorofluorescein and 8-isoprostane in the brain. Hepcidin expression was detected by real-time PCR and immunofluorescence analysis. Iron contents were measured using Perl's staining as well as graphite furnace atomic absorption spectrophotometry. Dichlorofluorescein and 8-isoprostane were determined using a fluorescence spectrophotometer and an ELISA kit, respectively. We found that hepcidin contents in the cortex, hippocampus, striatum and substantia nigra of rats treated with ad-hepcidin are 3.50, 2.98, 2.93 and 4.07 fold of those of the control rats respectively. Also, we demonstrated that the increased iron as well as dichlorofluorescein and 8-isoprostane levels in all four brain regions, induced by injection of iron dextran, could be effectively prevented by pre-treatment of the rats with ad-hepcidin. We concluded that pre-treatment with ad-hepcidin could increase hepcidin expression and prevent the increase in iron and reduce reactive oxygen species in the brain of iron-overloaded rats. (C) 2015 Elsevier Inc. All rights reserved.