文献类型：期刊文献

英文题名：Numerical and experimental assessment of a miniature bioreactor equipped with a mechanical agitator and non-invasive biosensors

作者：Li, Chao[1];Tian, Jiangtao[1];Wang, Weifei[1];Peng, Huadong[2,3];Zhang, Ming[1];Hang, Haifeng[1];Zhang, Siliang[1];Xia, Jian-Ye[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Imperial Coll London, Dept Bioengn, London, England;[3]Imperial Coll London, Ctr Synthet Biol, London, England

年份：2019

卷号：94

期号：8

起止页码：2671

外文期刊名：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20192507072957);WOS:【SCI-EXPANDED(收录号:WOS:000475479300028)】；

基金：This work was supported by the Fundamental Research Funds for the Central Universities (grant number 22221818014), the National Natural Science Foundation of China (grant number: 21776082, 21506052) and the Programme of Introducing Talents of Discipline to Universities (grant number B18022).

语种：英文

外文关键词：miniature bioreactor; computational fluid dynamics; mass transfer; shear rate; mixing

摘要：BACKGROUND Sufficient oxygen supply and stable process control are crucial for the successful screening and process optimization of aerobic microorganisms in small-scale bioreactors. In this work, a miniature bioreactor (volume 80 mL) equipped with non-invasive biosensors was developed and characterized. RESULTS To enable proper mixing and high oxygen transfer rate, a mechanical agitator with large diameter (0.56 tank diameter) and submerged aeration were applied. The flow fields formed in the miniature bioreactor under a wide range of conditions (filling volume 30-70 mL, agitation speed 300-1100 rpm and aeration rate 0-4 vvm) were numerically analyzed by computational fluid dynamics (CFD). The miniature bioreactor performed with a suitable mixing time (<4 s) and a high oxygen transfer coefficient (k(L)a > 1000 h(-1)). It showed that engineering parameters including mass transfer, mixing and shear were more sensitive to agitation than either filling volume or aeration. Correlations for quantitative evaluation of the engineering parameters were established. The predicted oxygen transfer coefficient by CFD showed good agreement with both experimental and reported data. Furthermore, cultivations of Escherichia coli and Pichia pastoris in the mini bioreactor verified the excellent performance. CONCLUSION The developed miniature bioreactor will provide a reliable tool for screening and process optimization, and the presented correlations that predict the engineering parameters will make it convenient to achieve scale-up. (c) 2019 Society of Chemical Industry