文献类型：期刊文献

英文题名：Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea)

作者：Wei, Wei[1];Wu, Haizhen[1];Xu, Hongyan[1];Zhang, Xiao[1];Chu, Teng[1];Wu, Changwen[2];Chang, Kangmei[2];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Ocean Univ, Coll Ocean Sci, Zhoushan 316004, Zhejiang Prov, Peoples R China

年份：2010

卷号：28

期号：1

起止页码：221

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000274235100028)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2006AA09Z436) and the Chinese Postdoctoral Science Foundation (20060400172).

语种：英文

外文关键词：Large yellow croaker (Pseudosciana crocea); Vibrio anguillarum; lycC1INH; Immune response

摘要：Complement component 1 inhibitor (C1INH) is a crucial protein in controlling activation of many plasma mediator pathways and can directly interact with Gram negative bacteria. The full-length cDNA of lycC1INH gene was identified from the large yellow croaker. It is of 2046 nucleotides (nt) encoding a protein of 599 amino acids, with a 5'-untranslated region of 99 nt and a 3'-untranslated region of 147 nt including the poly (A) tail. The deduced protein contains a C-terminal serpin (serine protease inhibitor) domain, and two N-terminus immunoglobulin domains without significant homology to other species. Western blot analysis of the protein expression showed that the expression of lycC1INH was obviously up-regulated in liver, spleen and head kidney of the fish challenged by attenuated live Vibrio anguillarum strain. This indicated that lycC1INH might be involved in the immune response of large yellow croaker to bacterial challenge. (C) 2009 Elsevier Ltd. All rights reserved.