文献类型：期刊文献

英文题名：Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering

作者：Li, Da-Wei[1,2];Qu, Lu-Lu[1,2];Hu, Kai[1,2];Long, Yi-Tao[1,2];Tian, He[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：54

期号：43

起止页码：12758

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20153601235426);WOS:【SCI-EXPANDED(收录号:WOS:000363422700045)】；

基金：This research was supported by NSFC (21421004, 21575041, 21125522, 21327807), the Shanghai Pujiang Program Grant of China (12JC1403500), the Shanghai Municipal Natural Science Foundation (14ZR1410800), and the Fundamental Research Funds for the Central Universities (WB1113005). The authors declare no competing financial interest.

语种：英文

外文关键词：hydrogen sulfide; living cells; monitoring; nanosensors; surface-enhanced Raman spectroscopy

摘要：Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nano-sensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2S. Furthermore, AuNPs/4-AA responds to H2S within 1 min with a 0.1 mu m level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2S.