文献类型：期刊文献

中文题名：高密度培养大肠杆菌TB1/pMAL-hOCIFm的优化条件

英文题名：High Cell-Density Culture of E.coli TB1/pMAL-hOCIFm

作者：张兴群[1];王梁华[2];焦炳华[2];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]第二军医大学基础医学部生化与分子生物学教研室,上海200433

年份：2005

卷号：31

期号：1

起止页码：72

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2005149026104);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(30000078);国家"863"计划资助项目(2001AA215441)

语种：中文

中文关键词：破骨细胞形成抑制因子(OCIF);表达;高密度培养;发酵;补料-分批培养

外文关键词：OCIF; expression; high density culture; fermentation; fed-batch

摘要：利用Bioengeering3.7L自控式发酵罐,以分批培养和补料分批培养相结合的培养技术,高密度培养重组大肠杆菌TB1/pMAL-hOCIFm,生产重组人破骨细胞形成抑制因子成熟肽(recombinanthumanosteoclastogenesisinhibitoryfactormaturepeptide,rhOCIFm)。通过对碳、氮源补加方式以及溶解氧等参数的控制,使工程菌E.coliTB1/pMAL-hOCIFm的发酵菌体OD600达到57.8,rhOCIFm与MBP融合蛋白(hOCIFm-MBP)的含量达到3.2g/L。本研究为工业化生产rhOCIFm奠定了基础。
Batch and fed-batch culture process of E. coli TB1/pMAL-hOCIFm were carried out to produce recombinant human osteoclastogenesis inhibitory factor mature peptide (rhOCIFm) in 3.7 L Bioengineering autocontrol fermentor. High cell-density and high expression were achieved by the control of carbon/nitrogen source and dissolved oxygen. The final cell density and concentration of rhOCIFm-MBP fusion protein were 57.8 OD600 and 3.2 g/L respectively. The purity of rhOCIFm-MBP fusin protein was more than 70% after treated with affinity chromatography on amylose resin. This study provides a basic work for the production of rhOCIFm in industrial scale.