文献类型：期刊文献

英文题名：Antibacterial and antibiofilm activities of eugenol from essential oil of Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf against periodontal pathogen Porphyromonas gingivalis

作者：Zhang, Yi[1];Wang, Yue[1,2];Zhu, Xiaojing[1];Cao, Ping[3];Wei, Shaomin[3];Lu, Yanhua[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Box 283,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Inst Technol, Shanghai 201418, Peoples R China;[3]Shanghai Jahwa Grp Co Ltd, Shanghai 200082, Peoples R China

年份：2017

卷号：113

起止页码：396

外文期刊名：MICROBIAL PATHOGENESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418631900055)】；

基金：This work is financially supported by Shanghai Jahwa (Group) Co., Ltd., as well as the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：Eugenol; Porphyromonas gingivalis; Cell membrane; Biofilm formation

摘要：The antibacterial effect and mechanism of eugenol from Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf essential oil (CLEO) against oral anaerobe Porphyromonas gingivalis were investigated. The results showed that eugenol, with content of 90.84% in CLEO, exhibited antibacterial activity against P. gingivalis at a concentration of 31.25 M. Cell shrink and lysis caused by eugenol were observed with Scanning Electron Microscopy (SEM). The release of macromolecules and uptake of fluorescent dye indicated that the antibacterial activity was due to the ability of eugenol to permeabilize the cell membrane and destroy the integrity of plasmatic membrane irreversibly. In addition, eugenol inhibited biofilm formation and reduced preformed biofilm of P. gingivalis at different concentrations. The down-regulation of virulence factor genes related to biofilm (fimA, hagA, hagB, rgpA, rgpB, kgp) explained that eugenol suppressed biofilm formation at the initial stage. These findings suggest that eugenol and CLEO may be potential additives in food and personal healthcare products as a prophylactic approach to periodontitis.