文献类型：期刊文献

中文题名：细菌外膜囊泡包覆的载药纳米粒的制备及其小鼠鼻腔免疫效果评价

英文题名：Preparation of bacterial outer membrane vesicle coated nanoparticle loaded with drug and evaluation of its nasal immune effect in mice

作者：胡慧冰[1];侯昕宇[1];贺牧野[1];高峰[1,2,3]

机构：[1]华东理工大学药学院药剂教研组;[2]上海市新药设计重点实验室;[3]上海市功能性材料化学重点实验室,上海200237

年份：2018

卷号：38

期号：2

起止页码：155

中文期刊名：上海交通大学学报（医学版）

外文期刊名：Journal of Shanghai Jiao tong University:Medical Science

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：上海市自然科学基金(17ZR1406600)~~

语种：中文

中文关键词：细菌外膜囊泡;卵清蛋白;聚乳酸-羟基乙酸共聚物;免疫;纳米给药系统

外文关键词：bacterial outer membrane vesicle;ovalbumin;poly(lactic-co-glycolic acid) copolymer;immunization;nanoparticle drug delivery system

摘要：目的·制备细菌外膜囊泡(outer membrane vesicle,OMV)包覆的载卵清蛋白(ovalbumin,OVA)聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid)copolymer,PLGA]纳米载体并用于小鼠鼻腔免疫效果评价。方法·采用超滤离心法制备OMV,采用乳化溶剂挥发法制备包载OVA的PLGA纳米粒(nanoparticle,NP),采用机械挤出法制备OMV包覆的PLGA载药NP,并对其进行表征。载OVA的OMV-PLGA NP经BALB/c小鼠鼻腔给药,用ELISA法检测鼻腔灌洗液、空肠黏膜和粪便中特异性sIgA抗体水平。结果·OMV包覆的PLGA载药NP粒径为(234.4±22.9)nm,透射电子显微镜观察其具有核壳结构。给药14 d后,载OVA的OMV-PLGA NP给药组在鼻腔灌洗液、空肠黏膜及粪便中sIgA抗体水平最高;与OMV+OVA给药组相比,载OVA的OMV-PLGA NP给药组在鼻腔灌洗液、空肠黏膜及粪便中的OVA特异性sIgA抗体水平分别提高了1.6、2.1和1.7倍,OMV特异性sIgA抗体水平均提高了1.5倍。结论·该纳米给药系统能同时使OMV和OVA被摄取与呈递,产生较强的小鼠黏膜免疫诱导反应。
Objective·To prepare a bacterial outer membrane vesicle(OMV) coated poly(lactic-co-glycolic acid) copolymer(PLGA) nanoparticle loaded with ovalbumin(OVA) and evaluate its intranasal immune effect in mice. Methods·OMV was prepared by ultrafiltration concentration method. OVA loaded PLGA nanoparticle(NP) was prepared by emulsion-solvent evaporation method. OMV coated PLGA nanoparticle(OMV-PLGA NP) loaded with OVA was prepared by extrusion method and characterized. BALB/c mice were intranasally immunized and specific sIgA levels in nasal wash, jejunum and fecal pellet were determined by ELISA. Results·Size of OVA loaded OMV-PLGA NP was(234.4±22.9) nm. The shell-core structure of OVA loaded OMV-PLGA NP was proved by transmission electron microscope. After 14 d of administration, sIgA antibody levels in nasal wash, jejunum and fecal pellet of OVA loaded OMV-PLGA NP treated group were the highest in all treated groups. Compared with the group treated with OMV and OVA, OVA-specific sIgA antibody level in nasal wash, jejunum and fecal pellet of OVA loaded OMV-PLGA NP treated group was increased 1.6, 2.1 and 1.7 times, respectively. Compared with the group treated with OMV and OVA, OMV-specific sIgA antibody level in nasal wash, jejunum and fecal pellet of OVA loaded OMV-PLGA NP treated group was all increased 1.5 times. Conclusion·This novel nanoparticle drug delivery system can simultaneously delivery OVA and OMV to antigen presenting cells, resulting in stronger mucosal immune response in mice.