文献类型：期刊文献

英文题名：High throughput pretreatment of corn stover using compacted biomass with recycled ammonia (COBRA) process

作者：Zhang, Jian[1];Mohammadi, Maedeh[2];Gong, Heng[1];Hodge, David B.[3];Tumuluru, Jayashankar[4];Sousa, Leonardo da Costa[5,6];Dale, Bruce[5,6];Balan, Venkatesh[2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Univ Houston, Cullen Coll Engn, Dept Engn Technol, Biotechnol Program, Sugar Land, TX 77479 USA;[3]Montana State Univ, Dept Chem & Biol Engn, Bozeman, MT 59717 USA;[4]USDA ARS, Southwestern Cotton Ginning Res Lab, 300 Coll Ave, Las Cruces, NM 88001 USA;[5]Michigan State Univ, Dept Chem Engn & Mat Sci, E Lansing, MI 48824 USA;[6]Michigan State Univ, Great Lakes Bioenergy Res Ctr GLBRC, Dept Energy DOE, E Lansing, MI 48824 USA

年份：2025

卷号：505

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20250417737232);WOS:【SCI-EXPANDED(收录号:WOS:001407418700001)】；

基金：This project was supported by Michigan Translational Research and Commercialization (MTRAC) Innovation Hub for AgBio, co-funded by the Michigan Economic Development Corporation (MEDC) through the Michigan Strategic Fund. Dr. Zhang would like to thank Natural Science Foundation of China (22178105) for supporting this research and travel to Michigan State University where the research work was carried out. Dr. Dale thanks the DOE Great Lakes Bioenergy Research Center (https:// www.greatlakesbioenergy.org) , funded by the U.S. Department of Energy through Cooperative Agreement DE-FC02-07ER64494, as well as Michigan State University AgBioResearch and the National Institute for Food and Agriculture of the U.S. Department of Agriculture #2021-67022-34889. Dr. Balan would like to thank the University of Houston and the state of Texas for his startup funds.

语种：英文

外文关键词：COBRA pretreatment; Ammonia-based pretreatment; Lignocellulosic biomass; Bioethanol; Cellulose crystallinity

摘要：In this study, a Compacted Biomass with Recycled Ammonia (COBRA) pretreatment was used, utilizing densified corn stover and anhydrous liquid ammonia under mild conditions to increase pretreatment throughput. COBRA was then compared with established Ammonia Fiber Expansion (AFEX) and Extractive Ammonia (EA) pretreatments for sugar conversion and ethanol fermentation. The optimal pretreatment conditions for the COBRA process were determined to be 67 degrees C, with an NH3-to-biomass ratio of 0.8:1, and a duration of 5.5 h. X-ray diffraction analysis confirmed that COBRA effectively converted recalcitrant crystalline cellulose I to the more digestible cellulose III. Using an optimized enzyme cocktail (CTec2:HTec2:Pectinex; 0.6:0.2:0.2) of 15 mg/g glucan, COBRA-pretreated corn stover achieved high sugar yields. Separate hydrolysis and fermentation (SHF) at 6 % glucan loading, yielded 62 g/L glucose and 28 g/L xylose after 96 h of hydrolysis. Subsequent fermentation with S. cerevisiae 424A for 96 h, without nutrient supplementation or detoxification, produced 38.3 g/L ethanol with an 87.6 % metabolic yield. COBRA pretreatment followed by hydrolysis and fermentation produced 216.9 g of ethanol per kg of biomass, compared to AFEX (198.0 g) and EA (235.9 g). While COBRA's ethanol yield was lower than EA, its advantages lie in improved reactor filling ratio (16.7 % vs 4.4 %), milder conditions (67 degrees C vs 120 degrees C, 400 psi vs 1300 psi), and lower ammonia usage (NH3:biomass 0.8:1 vs 6:1). A techno-economic analysis showed that integrating COBRA pretreatment with a localized biomass supply chain system is economically viable for large-scale cellulosic ethanol production.