文献类型：期刊文献

英文题名：A novel quadruplex real-time PCR method for simultaneous detection of Cry2Ae and two genetically modified cotton events (GHB119 and T304-40)

作者：Li, Xiang[1];Wang, Xiuxiu[2];Yang, Jielin[1];Liu, Yueming[1];He, Yuping[1];Pan, Liangwen[1]

机构：[1]GMO Detect Lab, Shanghai Entry Exit Inspect & Quarantine Bur, Shanghai 200135, Peoples R China;[2]E China Univ Sci & Technol, Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2014

卷号：14

外文期刊名：BMC BIOTECHNOLOGY

收录：MEDLINE(收录号：24884946);;EI(收录号：20143118002085);WOS:【SCI-EXPANDED(收录号:WOS:000338143000001)】；PubMed;

基金：We thank Dr. Litao Yang and Jianxin Shi of Shanghai Jiao Tong University for their kind help in revising of the vocabulary and grammar of the manuscript. This work was supported by grants from the Research Fund of General Administration of Quality Supervision, Inspection and Quarantine of China (2012IK185), the Shanghai Public Service Platform Special Fund (13DZ2290700) and the Science and Technology Program of Shanghai Entry-Exit Inspection and Quarantine Bureau (HK004-2012).

语种：英文

外文关键词：Cry2Ae; Event-specific; GHB119; Quadruplex real-time PCR; T304-40

摘要：Background: To date, over 150 genetically modified (GM) crops are widely cultivated. To comply with regulations developed for genetically modified organisms (GMOs), including labeling policies, many detection methods for GMO identification and quantification have been developed. Results: To detect the entrance and exit of unauthorized GM crop events in China, we developed a novel quadruplex real-time PCR method for simultaneous detection and quantification of GM cotton events GHB119 and T304-40 in cotton-derived products (based on the 5'-flanking sequence) and the insect-resistance gene Cry2Ae. The limit of detection was 10 copies for GHB119 and Cry2Ae and 25 copies for T304-40. The limit of quantification was 25 copies for GHB119 and Cry2Ae and 50 copies for T304-40. Moreover, low bias and acceptable standard deviation and relative standard deviation values were obtained in quantification analysis of six blind samples containing different GHB119 and T304-40 ingredients. Conclusions: The developed quadruplex quantitative method could be used for quantitative detection of two GM cotton events (GHB119 and T304-40) and Cry2Ae gene ingredient in cotton derived products.