文献类型：期刊文献

中文题名：Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

英文题名：Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

作者：Yuanting LI[1];Dawei LI[1];Wei SONG[1];Meng LI[1];Jie ZOU[2];Yitao LONG[1]

机构：[1]Key Laboratory for Advanced Materials&Department of Chemistry,East China University of Science and Technology,Shanghai 200237,China;[2]Jiangsu Provincial Supervising&Testing Research Institute for Products Quality,Nanjing 210007,China

年份：2012

卷号：6

期号：6

起止页码：831

中文期刊名：Frontiers of Environmental Science & Engineering

外文期刊名：环境科学与工程前沿（英文）

收录：Scopus;CSCD:【CSCD2011_2012】；

语种：英文

中文关键词：on-site determination;tyrosinase biosensor,phenolic contaminants;single-walled carbon nanotubes,gold nanoparticles;screen-printed electrodes

外文关键词：on-site determination, tyrosinase biosensor,phenolic contaminants, single-walled carbon nanotubes,gold nanoparticles, screen-printed electrodes

摘要：A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase （SWCNTs-AuNPs-Tyr） modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron micro- scopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase （Tyr） with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10^-8 to 2.0 × 10^- 5 mol.L-1 with a detection limit of 4.5 × 10^-8 mol.L-1 （S/N= 3）. This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosen- sor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.
A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase （SWCNTs-AuNPs-Tyr） modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron micro- scopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase （Tyr） with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10^-8 to 2.0 × 10^- 5 mol.L-1 with a detection limit of 4.5 × 10^-8 mol.L-1 （S/N= 3）. This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosen- sor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.