文献类型：期刊文献

英文题名：Insights into Partial Nitrification in a Membrane-Aerated Biofilm Reactor (MABR): Performance, Microbial Characteristics, and Mechanisms

作者：Yan, Zixuan[1,2];Han, Xushen[1,2];Lin, Yuqing[1,2];Jin, Yan[2];Song, Xingfu[1,2]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asses, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Natl Engn Res Ctr Integrated Utilizat Salt Lake Re, Shanghai 200237, Peoples R China

年份：2025

卷号：5

期号：10

起止页码：2555

外文期刊名：ACS ES&T ENGINEERING

收录：WOS:【ESCI(收录号:WOS:001526043300001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (52300085), the Shanghai Sailing Program (20YF1409500), and the Fundamental Research Funds for the Central Universities (JKB01241707).

语种：英文

外文关键词：membrane-aerated biofilm reactor(MABR); aeration pressure; partial nitrification; nitrifying bacteria; biofilm structure

摘要：Membrane-aerated biofilm reactor (MABR) enables molecular oxygen diffusion via gas-permeable membranes, offering potential for accurate partial nitrification (PN) manipulation with highly efficient oxygen utilization. To date, the PN-MABR has remained unstable/unsatisfactory, and the mechanisms governing oxygen transfer and microbial distribution within biofilms are still unclear. To clarify these mechanisms, three MABRs were operated under extremely low aeration pressures (R1, 0.4 kPa; R2, 0.6 kPa; R3, 0.8 kPa) to initiate PN in high-strength nitrogen wastewater (200 mg/L NH4 +-N). Highly efficient NH4 +-N removal (82.8%) and NO2 --N accumulation (86.2%) were achieved at 0.6 kPa with the enrichment of ammonia-oxidizing bacteria (AOB) and archaea (AOA), surpassing results from prior PN-MABR studies. RT-qPCR and fluorescence in situ hybridization (FISH) analyses indicated the unavoidable presence of nitrite-oxidizing bacteria (NOB) and oxygen-rich zones within the inner MABR biofilm. The oxygen affinity and oxygen transfer coefficient of the membrane could affect oxygen levels at the membrane-biofilm interface, owing to high oxygen concentrations (0.94, 3.40, and 3.52 mg/L) at the membrane-biofilm interface under the three aeration pressures, based on theoretical calculations. Therefore, entire NOB suppression could not be realized by controlling the oxygen supply alone. Further studies should focus on membrane materials with lower oxygen affinity to achieve higher PN efficiency.