文献类型：期刊文献

英文题名：Long-chain n-alkane biodegradation coupling to methane production in an enriched culture from production water of a high-temperature oil reservoir

作者：Chen, Jing[1,2,6];Liu, Yi-Fan[1,2,6];Zhou, Lei[1,2,6];Irfan, Muhammad[1,2,5];Hou, Zhao-Wei[3];Li, Wei[3];Mbadinga, Serge Maurice[1,2,6];Liu, Jin-Feng[1,2,6];Yang, Shi-Zhong[1,2,6];Wu, Xiao-Lin[3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Daqing Oilfield Co Ltd, Res Inst, PetroChina, Daqing 163712, Heilongjiang, Peoples R China;[4]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[5]Univ Engn & Technol, Dept Chem Polymer & Composite Mat Engn, KSK Campus, Lahore 54890, Pakistan;[6]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：AMB EXPRESS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000525264100001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grants No. 41530318, 41373070, 41807324), NSFC/RGC Joint Research Fund (No. 41161160560), the Shanghai International Collaboration Program (18230743300), the Fundamental Research Funds for the Central Universities (No. 222201817017, 50321101917017, 22221818014) and the Research Program of State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Anaerobic biodegradation; Long-chain n-alkanes; High-temperature oil; petroleum reservoir; Methanogenesis; Metabolic pathway

摘要：Paraffinic n-alkanes (C22-C30), crucial portions of residual oil, are generally considered to be difficult to be biodegraded owing to their general solidity at ambient temperatures and low water solubility, rendering relatively little known about metabolic processes in different methanogenic hydrocarbon-contaminated environments. Here, we established a methanogenic C22-C30 n-alkane-degrading enrichment culture derived from a high-temperature oil reservoir production water. During two-year incubation (736 days), unexpectedly significant methane production was observed. The measured maximum methane yield rate (164.40 mu mol L-1 d(-1)) occurred during the incubation period from day 351 to 513. The nearly complete consumption (> 97%) of paraffinic n-alkanes and the detection of dicarboxylic acids in n-alkane-amended cultures indicated the biotransformation of paraffin to methane under anoxic condition. 16S rRNA gene analysis suggested that the dominant methanogen in n-alkane-degrading cultures shifted from Methanothermobacter on day 322 to Thermoplasmatales on day 736. Bacterial community analysis based on high-throughput sequencing revealed that members of Proteobacteria and Firmicutes exhibiting predominant in control cultures, while microorganisms affiliated with Actinobacteria turned into the most dominant phylum in n-alkane-dependent cultures. Additionally, the relative abundance of mcrA gene based on genomic DNA significantly increased over the incubation time, suggesting an important role of methanogens in these consortia. This work extends our understanding of methanogenic paraffinic n-alkanes conversion and has biotechnological implications for microbial enhanced recovery of residual hydrocarbons and effective bioremediation of hydrocarbon-containing biospheres.