文献类型：期刊文献

英文题名：Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

作者：Zhang, Li-xiang[1]; Zhang, Wei-yan[1]; Wang, Chen[1]; Liu, Jin-tao[1]; Deng, Xian-cun[1,2]; Liu, Xu-ping[1]; Fan, Li[1]; Tan, Wen-song[1]

机构：[1] The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, Shanghai, 200237, China; [2] Zhejiang Hisun Pharmaceutical [Hangzhou] Co. Ltd, Fuyang, Hangzhou, Zhejiang, 311404, China

年份：2016

卷号：3

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513075909)

语种：英文

外文关键词：Amino acids - Cell culture - Cell growth - Growth kinetics - Monoclonal antibodies - Physiology

摘要：Background: Optimization of chemically defined medium has been a critical way to produce monoclonal antibody. Usually, amount of glutamine was added into the feed medium, but a half of asparagine was added. Our study found that asparagine was important in the antibody production phase. Increasing the ratio of asparagine to glutamine in feed medium for enhancement of antibody production in CHO-DHFR cell culture would be an efficient way. Results: We optimized the total amount and the ratio of the two vital amino acids in feed medium to increase antibody production. In this work, we have demonstrated that feeding medium of high ratio between asparagine and glutamine (FB-H) can enhance the cell density after reaching the stationary phase. Moreover, FB-H was shown to improve cell maintenance, and increased the antibody production. The metabolic flux analysis proved that ratio of asparagine to glutamine had little influence on glycolysis. Furthermore, the TCA cycle of FB-H was enhanced by 20% compared to that of low ratio of asparagine to glutamine (FB-L). And the energy metabolism of FB-H was 22.6% higher than that of FB-L. For the later, lactate can be less produced in FB-H. Conclusions: We should improve the ratio between asparagine and glutamine in feed medium properly under the premise of no influence on cell growth to achieve high mAb producing goal. ? 2016, Zhang et al.