文献类型：期刊文献

英文题名：Yarrowia lipolytica as a Metabolic Engineering Platform for the Production of Very-Long-Chain Wax Esters

作者：Gao, Qi[1];Yang, Jing-Lin[1];Zhao, Xin-Ru[1];Liu, Shun-Cheng[1];Liu, Zhi-Jie[2];Wei, Liu-Jing[1];Hua, Qiang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hubei Univ Technol, Hubei Collaborat Innovat Ctr Ind Fermentat, Hubei Key Lab Ind Microbiol, Key Lab Fermentat Engn,Minist Educ, Wuhan 430068, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2020

卷号：68

期号：39

起止页码：10730

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20204509469043);WOS:【SCI-EXPANDED(收录号:WOS:000577116200023)】；

基金：This study was financially supported by the National Natural Science Foundation of China (21776081) and the National Key R&D Program of China (2019YFA0904302).

语种：英文

外文关键词：Yarrowia lipolytica; very-long-chain fatty acids; fatty alcohols; wax esters

摘要：The oleaginous yeast Yarrowia lipolytica is an attractive cell factory platform strain and can be used for sustainable production of high-value oleochemical products. Wax esters (WEs) have a good lubricating property and are usually used as a base for the production of advanced lubricants and emollient oils. In this study, we reported the metabolic engineering of Y. lipolytica to heterologously biosynthesize high-content very-long-chain fatty acids (VLCFAs) and fatty alcohols and efficiently esterify them to obtain very-long-chain WEs. Co-expression of fatty acid elongases from different sources in Y. lipolytica could yield VLCFAs with carbon chain lengths up to 24. Combining with optimization of the central metabolic modules could further enhance the biosynthesis of VLCFAs. Furthermore, through the screening of heterologous fatty acyl reductases (FARs), we enabled high-level production of fatty alcohols. Genomic integration and heterologous expression of wax synthase (WS) and FAR in a VLCFA-producing Y. lipolytica strain yielded 95-650 mg/L WEs with carbon chain lengths from 32 to 44. Scaled-up fermentation in 5 L laboratory bioreactors significantly increased the production of WEs to 2.0 g/L, the highest content so far in yeasts. This study contributes to the further efficient biosynthesis of VLCFAs and their derivatives.