文献类型：期刊文献

英文题名：One-pot synthesis of aldehyde-functionalized mesoporous silica-Fe3O4 nanocomposites for immobilization of penicillin G acylase

作者：Yang, Ling[1];Guo, Yanglong[1];Zhan, Wangcheng[1];Guo, Yun[1];Wang, Yunsong[1];Lu, Guanzhong[1]

机构：[1]E China Univ Sci & Technol, Res Inst Ind Catalysis, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2014

卷号：197

起止页码：1

外文期刊名：MICROPOROUS AND MESOPOROUS MATERIALS

收录：;EI(收录号：20142717907619);WOS:【SCI-EXPANDED(收录号:WOS:000340990200001)】；

基金：This project was supported by National Basic Research Program of China (2013CB933201, 2010CB732300), National Natural Science Foundation of China (21103048), Program for New Century Excellent Talents in University (NCET-09-0343), Shu Guang Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (10SG30).

语种：英文

外文关键词：Mesoporous silica; Aldehyde groups; Paramagnetic; Penicillin G acylase; Immobilization

摘要：Aldehyde-functionalized mesoporous silica-Fe3O4 nanocomposites, one-pot synthesized by co-condensation of tetraethylorthosilicate and trimethoxysilylpropanal in presence of triblock copolymer of Pluronic P123, NaCl and Fe3O4 nanoparticles in the neutral solution, were characterized by SAXS, XRD, TEM, nitrogen sorption, FT-IR, TG, elementary analysis and magnetic susceptibility measurements, and investigated as the supports for covalent immobilization of penicillin G acylase (PGA). The results show that paramagnetic Fe3O4 nanoparticles were embedded among the cannular mesoporous silica layers and the aldehyde groups were condensed on the surface of mesoporous silica. PGA was covalently immobilized on these paramagnetic nanocomposites via the reaction to produce Schiffs base between the free amino groups of lysine residues of PGA and the aldehyde groups on the surface of these nanocomposites. PGA immobilized on these paramagnetic nanocomposites had better operational stability and was easily recycled by an external magnetic field. The immobilized PGA had the initial activity of 6231 U g(-1) and the operational stability of 91.0% of the initial activity after recycled for 10 times. (C) 2014 Elsevier Inc. All rights reserved.