文献类型：期刊文献

英文题名：Preparation and properties of BSA-loaded microspheres based on multi-(amino acid) copolymer for protein delivery

作者：Chen, Xingtao[1];Lv, Guoyu[1];Zhang, Jue[2];Tang, Songchao[2];Yan, Yonggang[1];Wu, Zhaoying[2];Su, Jiacan[2];Wei, Jie[2]

机构：[1]Sichuan Univ, Coll Phys Sci & Technol, Chengdu 610041, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai, Peoples R China

年份：2014

卷号：9

起止页码：1957

外文期刊名：INTERNATIONAL JOURNAL OF NANOMEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000209806600001)】；

基金：The authors are grateful for the financial support from the International Cooperation Project of the Ministry of Science and Technology of China (2013DFB50280), the National Natural Science Foundation of China (No 81271705, 51173041, 31271031), the Nano Special Program of Science and Technology Development of Shanghai (No 12nm0500400), and the Key Medical Program of Science and Technology Development of Shanghai (No 12441903600).

语种：英文

外文关键词：poly (amino acid) copolymer; release; degradation

摘要：A multi-(amino acid) copolymer (MAC) based on omega-aminocaproic acid, gamma-aminobutyric acid, L-alanine, L-lysine, L-glutamate, and hydroxyproline was synthetized, and MAC microspheres encapsulating bovine serum albumin (BSA) were prepared by a double-emulsion solvent extraction method. The experimental results show that various preparation parameters including surfactant ratio of Tween 80 to Span 80, surfactant concentration, benzyl alcohol in the external water phase, and polymer concentration had obvious effects on the particle size, morphology, and encapsulation efficiency of the BSA-loaded microspheres. The sizes of BSA-loaded microspheres ranged from 60.2 mu m to 79.7 mu m, showing different degrees of porous structure. The encapsulation efficiency of BSA-loaded microspheres also ranged from 38.8% to 50.8%. BSA release from microspheres showed the classic biphasic profile, which was governed by diffusion and polymer erosion. The initial burst release of BSA from microspheres at the first week followed by constant slow release for the next 7 weeks were observed. BSA-loaded microspheres could degrade gradually in phosphate buffered saline buffer with pH value maintained at around 7.1 during 8 weeks incubation, suggesting that micro-sphere degradation did not cause a dramatic pH drop in phosphate buffered saline buffer because no acidic degradation products were released from the microspheres. Therefore, the MAC microspheres might have great potential as carriers for protein delivery.