文献类型：期刊文献

英文题名：Molecular analysis of the bacterial community in a continental high-temperature and water-flooded petroleum reservoir

作者：Li, H; Yang, SZ; Mu, BZ; Rong, ZF; Zhang, J

机构：[1]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Bioengn, Shanghai 200237, Peoples R China

年份：2006

卷号：257

期号：1

起止页码：92

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000235938200013)】；

语种：英文

外文关键词：petroleum reservoir; 16S rRNA gene diversity; restriction fragment length polymorphism; bacterial community; clone library

摘要：Water from a continental high-temperature, long-term water-flooded petroleum reservoir in Huabei Oilfield in China was analysed for its bacterial community and diversity. The bacteria were characterized by their 16S rRNA genes. A 16S rRNA gene clone library was constructed from the community DNA, and using restriction fragment length polymorphism analysis, 337 randomly selected clones were clustered with 74 operational taxonomic units. Sequencing and phylogenetic analyses showed that the screened clones were affiliated with Gammaproteobacteria (85.7%), Thermotogales (6.8%), Epsilonproteobacteria (2.4%), low-G+C Gram-positive (2.1%), high-G+C Gram-positive, Betaproteobacteria and Nitrospira (each < 1.0%). Thermopilic bacteria were found in the high-temperature water from the flooded petroleum reservoir, as well as mesophilic bacteria such as Pseudomonas-like clones. The mesophilic bacteria were probably introduced into the reservoir as it was being exploited. This work provides significant information on the structure of bacterial communities in high-temperature, long-term water-flooded petroleum reservoirs.