文献类型：期刊文献

英文题名：Preparation and characterization of a pH-responsive mesoporous silica nanoparticle dual-modified with biopolymers

作者：Li, Xiaoran[1];Garamus, Vasil M.[2];Li, Na[3,4];Gong, Yabin[5];Zhe, Zhe[6];Tian, Zhenfen[1];Zou, Aihua[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Helmholtz Zentrum Geesthacht, Inst Mat Res, Ctr Mat & Coast Res, Max Planck Str 1, D-21502 Geesthacht, Germany;[3]Natl Ctr Prot Sci Shanghai, Shanghai 200237, Peoples R China;[4]Shanghai Inst Biochem & Cell Biol, Shanghai 200237, Peoples R China;[5]Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai 200237, Peoples R China;[6]Shanghai Univ Tradit Chinese Med, Shanghai Municipal Hosp Tradit Chinese Med, Shanghai 200237, Peoples R China

年份：2018

卷号：548

起止页码：61

外文期刊名：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

收录：;EI(收录号：20181404972859);WOS:【SCI-EXPANDED(收录号:WOS:000430039800009)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31200617), Shanghai Natural Science Foundation (No. 15ZR1409900), and Knowledge Innovation Program of CAS (No. 2013KIP103). Thanks to the staff of the BL19U2 beamline at the National Center for Protein Science Shanghai and the Shanghai Synchrotron Radiation Facility for assistance during data collection.

语种：英文

外文关键词：MSNs; PEG; PDA; Highly dispersed; SAXS

摘要：This study was aimed at preparing a highly dispersed and pH-sensitive mesoporous silica nanoparticles (MSNs) carrier via surface modification with polydopamine (PDA) and polyethylene glycol (PEG) i.e. MSNs@PDA-PEG. Small angle X-ray scattering (SAXS) and transmission electron microscopy (TEM) measurements suggested that hexagonally structured MSNs (type MCM-41) with the layer of PDA and PEG of thickness similar to 20 nm were successfully prepared. Bovine serum protein (BSA) adsorption experiments showed that less protein was absorbed by MSNs@PDA-PEG after the anchoring of PEG, which confirmed the stealth properties of prepared carriers. Doxorubicin hydrochloride (DOX) was used as the model drug to be encapsulated into MSNs@PDA-PEG (DOX/MSNs@PDA-PEG). The DOX/MSNs@PDA-PEG had relatively high entrapment efficiency and drug loading up to 94.2% and 31.4%, respectively. The in vitro release profile of DOX/MSNs@PDA-PEG exhibited pH-responsive and gradual drug release. Cytotoxicity assay showed that DOX/MSNs@PDA-PEG had comparable cytotoxicity to A549 cells compared to pure DOX. The intracellular distribution of DOX/MSNs@PDA-PEG was imaged using confocal laser scanning microscope. It points that PDA and PEG can play "gatekeeper" and stealth roles for MSNs as promising nanocarrier for cancer treatment.