文献类型：期刊文献

英文题名：Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003

作者：Wang, Shu[1,4];Mao, Xiangzhao[2];Wang, Hualei[3];Lin, Jinping[3];Li, Fuli[1];Wei, Dongzhi[3]

机构：[1]Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China;[2]Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

年份：2011

卷号：91

期号：2

起止页码：287

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20113014187973);WOS:【SCI-EXPANDED(收录号:WOS:000292203200006)】；

基金：Financial support from the National Science Foundation of China (30900025) and National Basic Research Program of China (973 Program, 2009CB724703) are gratefully acknowledged.

语种：英文

外文关键词：Dextran; Gluconobacter oxydans; Monosaccharide composition; Structure analysis

摘要：A novel water-soluble dextran was synthesized from maltodextrin by cell-free extract of Gluconobacter oxydans DSM 2003. The dextran was purified by size exclusion chromatography, and the structure was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and gas chromatography-mass spectrometer. Based on the spectral data, we found that the dextran contained only d-glucose residues. The ratio of nonreducing end glucopyranosyl (Glcp) to 6-linked Glcp to 4,6-linked Glcp was estimated to be 8.62:78.79:12.59 by methylation analysis. This result indicated the existence of a small proportion of alpha(1,4) branches in alpha(1,6) glucosyl linear chains. Here, we reported the first time a novel dextran was synthesized by G. oxydans DSM 2003.