文献类型：期刊文献

英文题名：Photosensitive Hydrogel Creates Favorable Biologic Niches to Promote Spinal Cord Injury Repair

作者：Cai, Zhengwei[1];Gan, Yibo[2,3];Bao, Chunyan[1];Wu, Wanjiang[4,5];Wang, Xuebin[1];Zhang, Zetong[2];Zhou, Qiang[2,6];Lin, Qiuning[1];Yang, Yi[1];Zhu, Linyong[1]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Army Med Univ, Natl & Reg United Engn Lab Tissue Engn, Dept Orthoped, Southwest Hosp,Third Mil Med Univ, 29 Gao Tan Yan St, Chongqing 400038, Peoples R China;[3]Army Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Third Mil Med Univ, 30 Gao Tan Yan St, Chongqing 400038, Peoples R China;[4]Army Med Univ, Natl Dept Neurosurg, Southwest Hosp, Third Mil Med Univ, 29 Gao Tan Yan St, Chongqing 400038, Peoples R China;[5]Army Med Univ, Key Lab Neurotrauma, Southwest Hosp, Third Mil Med Univ, 29 Gao Tan Yan St, Chongqing 400038, Peoples R China;[6]Chongqing Med Univ, Bone & Trauma Ctr, Affiliated Hosp 3, Gener Hosp, Chongqing 401120, Peoples R China

年份：2019

卷号：8

期号：13

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000475931000011)】；

基金：Z.C. and Y.G. contributed equally to this work. This work was supported by grants from the NSFC of China (Grant Nos. 21472044, 81802165, and 81772378), and the Key Project of Military Medical Technology Innovation Program (Grant No. SWH2017JCZD-02).

语种：英文

外文关键词：hydrogels; nerve guidance conduits; photochemistry; protein immobilization; spinal cord injury repair

摘要：Photochemistry is considered to be a promising strategy for hydrogels to mimic the complex and dynamic properties of natural extracellular matrix. However, it is seldom applied in 3D tissue engineering and regenerative medicine due to the attenuation of light. In this study, phenyl azide photchemistry and optical fiber technology are first used to localize adhesive protein on the inner surface of the nerve guidance conduit in a 3D hydrogel scaffold. In vitro coculture assay of neural stem cells (NSCs) shows that photoimmobilization of collagen significantly improves the adhesion and survival of NSCs in the conduit, and exhibits synergistic effect with the sustainable release of growth factor. After implantation in transected spinal cord, the optimized hydrogel scaffold is found to improve the locomotion recovery of rats 12 weeks after spinal cord injury (SCI). Histological analysis suggests that the designed hydrogel scaffold provides a favorable biological niche for neuronal regeneration, thus producing directional neuron tissue and promoting the repair of SCI. This study demonstrates a promising hydrogel scaffold for SCI repair and provides the first understanding of the photoimmobilization of adhesive protein in a 3D hydrogel conduit concerning its functions on spinal cord tissue restoration.