文献类型：期刊文献

英文题名：Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast

作者：Ma, Ke[1];Deng, Lei[1];Wu, Haizhen[1,2];Fan, Jianhua[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China

年份：2022

卷号：9

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513079107);WOS:【SCI-EXPANDED(收录号:WOS:000840318200002)】；

基金：This work was supported by National Key Research and Development Project of China (2019YFA0906300 and 2020YFA0907304), National Natural Science Foundation of China (31872608), Natural Science Foundation of Shandong Province (ZR2019ZD17), Natural Science Foundation of Shanghai (21ZR1416400), and Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Chlamydomonas reinhardtii; Chloroplast; Synthetic biology; Green factory; Selectable marker; Protein expression

摘要：Microalgae are cosmopolitan organisms in nature with short life cycles, playing a tremendous role in reducing the pressure of industrial carbon emissions. Besides, microalgae have the unique advantages of being photoautotrophic and harboring both prokaryotic and eukaryotic expression systems, becoming a popular host for recombinant proteins. Currently, numerous advanced molecular tools related to microalgal transgenesis have been explored and established, especially for the model species Chlamydomonas reinhardtii (C. reinhardtii hereafter). The development of genetic tools and the emergence of new strategies further increase the feasibility of developing C. reinhardtii chloroplasts as green factories, and the strong genetic operability of C. reinhardtii endows it with enormous potential as a synthetic biology platform. At present, C. reinhardtii chloroplasts could successfully produce plenty of recombinant proteins, including antigens, antibodies, antimicrobial peptides, protein hormones and enzymes. However, additional techniques and toolkits for chloroplasts need to be developed to achieve efficient and markerless editing of plastid genomes. Mining novel genetic elements and selectable markers will be more intensively studied in the future, and more factors affecting protein expression are urged to be explored. This review focuses on the latest technological progress of selectable markers for Chlamydomonas chloroplast genetic engineering and the factors that affect the efficiency of chloroplast protein expression. Furthermore, urgent challenges and prospects for future development are pointed out.