文献类型：期刊文献

英文题名：Ampicillin-induced resistant variants of Listeria monocytogenes: effects on growth, survival, and virulence

作者：Sun, Lu[1];Cai, Hua[2];Wu, Yufan[3];Hong, Yi[4];Li, Yun[1];Xia, Keer[1];Liang, Jiwei[1];Li, Zhuosi[1];Dong, Qingli[1];Wang, Xiang[1]

机构：[1]Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Jungong Rd 516, Shanghai 200093, Peoples R China;[2]Shanghai Municipal Ctr Dis Control & Prevent SCDC, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai, Peoples R China;[4]Univ Ghent, Fac Biosci Engn, Dept Food Technol Safety & Hlth, Food Microbiol & Food Preservat Res Unit, Ghent, Belgium

年份：2025

卷号：221

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20253919215474);WOS:【SCI-EXPANDED(收录号:WOS:001580855000005)】；

基金：This study was funded by the Intergovernmental International Sci-ence and Technology innovation cooperation key project of National Key R &D Program of China (2024YFE0102600) , the Shanghai Agricul-ture Applied Technology Development Program (2022 02 08 00 12 F01144) . The authors have not stated any conflicts of interest.

语种：英文

外文关键词：Foodborne pathogens; Antibiotic resistance; Phenotypic adaptation; Whole genome sequencing; SNP analysis

摘要：Listeria monocytogenes is a major foodborne pathogen whose rising antibiotic resistance poses increasing public health concerns. Understanding how resistance acquisition affects bacterial physiology is essential for accurate microbial risk assessment. In this study, five antibiotic-sensitive foodborne L. monocytogenes strains were subjected to ampicillin (AMP) exposure to obtain AMP-resistant variants. Paired susceptible and resistant strains were compared for growth kinetics at 25 degrees C, 30 degrees C, and 37 degrees C; tolerance to heat (60 degrees C) and acid stress (pH 2.5); biofilm formation; and in vivo virulence using a Galleria mellonella model. Whole-genome sequencing and SNP analysis identified resistance-associated mutations. The AMP-resistant strains exhibited cross-resistance to multiple antibiotic classes, including lipopeptides, lincosamides, tetracyclines, and penicillins. Phenotypically, these strains exhibited a higher growth rate but an extended lag phase at 30 degrees C, along with reduced thermotolerance, decreased acid resistance, diminished biofilm-forming capacity, and attenuated virulence in the infection model. Whole-genome sequencing (WGS) combined with single nucleotide polymorphism (SNP) analysis identified non-synonymous mutations in five genes (bgsB, pbpA, htrA, uhpT, and mepA), potentially underpinning these phenotypic shifts. These results suggest a trade-off between antibiotic resistance and other biological characteristics in L. monocytogenes, offering valuable insights for microbial risk assessment. Monitoring of resistance-related adaptations and integrating genomic data into food safety strategies will be essential for controlling antibiotics resistant pathogens in food chain and minimizing associated public health risks.