文献类型：期刊文献

英文题名：Laser transparent multiplexed SERS microneedles for in situ and real-time detection of inflammation

作者：Peng, Cheng[1];Jin, Lili[2];Wang, Feng[1];Yang, Haifeng[1];He, Hongyan[2]

机构：[1]Shanghai Normal Univ, Shanghai Frontiers Sci Ctr Biomimet Catalysis, Joint Int Res Lab Resource Chem, Shanghai Key Lab Rare Earth Funct Mat,Minist Educ,, Shanghai 200234, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2023

卷号：225

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20231113709214);WOS:【SCI-EXPANDED(收录号:WOS:000930964700001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China [21475088 and 21507087] ; the National Natural Science Foundation of China for Innovative Research Groups [Grant No. 51621002] , PCSIRT [IRT1269] , China; and the Shanghai Municipal Government [18DZ2254200] , China.

语种：英文

外文关键词：Inflammation; Microneedles; pH; Reactive oxygen species level; Redox potential; Surface-enhanced Raman scattering

摘要：It is a significant challenge to perform painless invasive detection of inflammation progression in relation to the evolution of pH, redox potential, and reactive oxygen species (ROS) levels in an in situ and real-time manner. In this work, polydopamine-modified, silver nanoparticle-decorated poly (methyl methacrylate) microneedles (AgNPs@PDA@MNs) have been developed as a multiplexed surface enhanced Raman scattering (SERS) diag-nostic platform. Using rhodamine 6G as the Raman signal molecule, the AgNPs@PDA@MNs demonstrated a significant enhancement with reasonable linearity in the range of 10-3-10-9 mol/L and the limit of detection is 1.0 x 10-10 mol/L 4-mercaptobenzoic acid, anthraquinone-2-carboxylic acid and para-aminothiophenol were covalently anchored on AgNPs@PDA@MNs SERS substrate. I1143/I1183, I1606/I1667 and I1143/I1077 were used as assay standards for pH, redox potential and ROS level detection, respectively. The SERS multiplexed transparent microneedles (SERS mtMNs) linearly responded to pH in the range of 4.0-8.0, redox potential in the range of 417.0-599.8 mV, and ROS levels in the range of 0-480 ng/mL, demonstrating a significant ability to detect complex inflammation in vivo, in situ and in real-time.