文献类型：期刊文献

英文题名：The development of a novel AND logic based fluorescence probe for the detection of peroxynitrite and GSH

作者：Sedgwick, Adam C.[1];Han, Hai-Hao[2,3];Gardiner, Jordan E.[1];Bull, Steven D.[1];He, Xiao-Peng[2,3];James, Tony D.[1]

机构：[1]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：9

期号：15

起止页码：3672

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20181705050771);WOS:【SCI-EXPANDED(收录号:WOS:000431389300006)】；

基金：We would like to thank the EPSRC and the University of Bath for funding. ACS and JEG thank the EPSRC for studentships. X.-P. He thanks the National Natural Science Foundation of China (21722801 and 21572058), the Science and Technology Commission of Shanghai Municipality (15540723800), the Fundamental Research Funds for the Central Universities (222201717003) and the Shanghai Rising-Star Program (16QA1401400) for financial support. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and ECUST for a guest professorship. The Catalysis and Sensing for our Environment (CASE) network is thanked for research exchange opportunities. NMR characterisation facilities were provided through the Chemical Characterisation and Analysis Facility (CCAF) at the University of Bath (http://www.bath.ac.uk/ccaf). The EPSRC UK National Mass Spectrometry Facility at Swansea University is thanked for analyses. All data supporting this study are provided as Electronic Supplementary Information (ESI) accompanying this paper.

语种：英文

外文关键词：Computer circuits - Fluorescence

摘要：We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO- and GSH (GSH-PF3). The GSH-PF3 probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, PF3. GSH-PF3 produced only a small fluorescence response towards the addition of GSH or ONOO- separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement. GSH-PF3 demonstrated an excellent selectivity towards both GSH and ONOO-. In cellular imaging experiments the probe was shown to be cell permeable with no 'turn-on' response observed for the addition of either GSH or ONOO- separately. However, in the presence of both analytes, a clear fluorescence response was observed in live cells. GSH-PF3 was further able to monitor the co-existence of metabolically produced ONOO- and GSH by exogenous stimulation.