文献类型：期刊文献

中文题名：新型类过氧化物纳米酶NC@MIL-100(Fe)的制备及其对生物硫醇的测定

英文题名：Preparation of a Novel Peroxide-like Nanozyme NC@MIL-100(Fe)and Its Application in Biothiols Analysis

作者：王佩瑶[1];张凌怡[1];张维冰[1]

机构：[1]华东理工大学化学与分子工程学院上海市功能性材料化学重点实验室,上海200237

年份：2022

卷号：41

期号：8

起止页码：1121

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央高校基本科研业务费专项资金资助项目(JKJ01211718)。

语种：中文

中文关键词：纳米酶;针状焦;MIL-100(Fe);谷胱甘肽;半胱氨酸

外文关键词：nanozyme;needle coke;MIL-100(Fe);glutathione;cysteine

摘要：该文利用针状焦(NC)与金属有机框架(MOFs)材料MIL-100(Fe)间的静电相互作用,制备了新型纳米酶NC@MIL-100(Fe),并将其应用于生物硫醇的分析。新材料不仅可充分利用针状焦固有的类酶活性,其片层结构也能够有效改善MIL-100(Fe)的团聚现象,提高材料的整体催化效果。与单独使用针状焦和MIL-100(Fe)作为纳米酶相比,NC@MIL-100(Fe)对底物的亲和力更强,催化反应速度更快,表现出良好的类过氧化物酶催化活性。以3,3′,5,5′-四甲基联苯胺(TMB)和H_(2)O_(2)为底物对其性能进行评价,得到的米氏常数(K_(m))分别为0.27 mmol/L和0.43 mmol/L,比辣根过氧化物酶小1.61倍和8.6倍。将该材料用于生物硫醇的分析,谷胱甘肽和半胱氨酸检测的线性范围分别为3~70μmol/L、1~80μmol/L,检出限分别为0.33μmol/L和0.22μmol/L。该法具有线性范围宽、检出限低且选择性良好的特点,用于人血清中生物硫醇浓度的检测取得了良好的效果。
Enzyme-catalyzed reactions are characteristic of high selectivity and high efficiency.Special?ly designed metal-organic frameworks(MOFs)have catalytic activities similar to natural enzymes,and their porous structures also provide a large number of active sites for catalytic reactions.In this paper,a novel nanozyme NC@MIL-100(Fe)was prepared based on the electrostatic interaction between needle coke(NC)and MOF material(MIL-100(Fe)),which was applied to the analytical methods develop?ment of biothiols.The inherent enzyme-like activity of NC was made full use for the new material,whose lamellar structure could effectively avoid the agglomeration phenomenon of MIL-100(Fe),improving the overall catalytic effect of the material.Compared with needle coke and MIL-100(Fe),NC@MIL-100(Fe)as nanozyme has a stronger affinity for the substrate and a faster reaction rate,thus showing a better peroxidase-like catalytic activity.Its performance was evaluated with 3,3′,5,5′-tetramethyl?benzidine(TMB)and H_(2)O_(2) as substrates,and the Michaelis constants(K_(m))obtained were 0.27 mmol/L and 0.43 mmol/L,respectively,which were 1.61 times and 8.6 times smaller than that of horseradish peroxidase.The material was applied to the development of analytical methods for glutathione and cyste?ine,with the linear ranges of 3-70μmol/L and 1-80μmol/L,and the detection limits of 0.33μmol/L and 0.22μmol/L,respectively,showing the advantages of wide linear range,low detec?tion limit and good selectivity.Furthermore,this material was successfully applied to the detection of biothiols concentration in human serum with satisfactory results.