文献类型：期刊文献

中文题名：Induction of Recombinant Uridine Phosphorylase and Its Application in Biosynthesis of Pyrimidine Nucleosides

英文题名：Induction of Recombinant Uridine Phosphorylase and Its Application in Biosynthesis of Pyrimidine Nucleosides

作者：Ding Qingbao[1];Ou Ling[1];We Dongzhi[1];We Xiaokun[2];Xu Yanmei[2];Zhang Chunyan[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Pucheng Biosci & Biotechnol Co Ltd, Shanghai 200235, Peoples R China

年份：2011

卷号：19

期号：1

起止页码：122

中文期刊名：Chinese Journal of Chemical Engineering

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：CSTPCD;;EI(收录号：20111113750270);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000288235600019)】；CSCD:【CSCD2011_2012】；

基金：Supported by "Production, Education & Research" item of Shanghai Baoshan (08-H-4).

语种：英文

中文关键词：5-fluorouridine; lactose; uridine phosphorylase; pyrimidine nucleoside; uridine; 5-fluorouracil

外文关键词：5-fluorouridine; lactose; uridine phosphorylase; pyrimidine nucleoside; uridine; 5-fluorouracil

摘要：Recombinant Escherichia coli pUDP,which overexpressed uridine phosphorylase（UPase）,was constructed.0.5 mmol·L 1lactose had a similar induction effect as the commonly used inducer IPTG during 2.5-5.5 h of cell growth.The lactose-induced UPase was stable at 50°C.Wet cells of pUDP was used as catalyst to biosynthesize 5-fluorouridine from 30 mmol·L 1uridine and 5-fluorouracil in phosphate buffer（pH 7.0）catalyzed at 50°C for 1.5 h and the yield of 5-fluorouridine was higher than 68%.Under the optimum reaction conditions for production of 5-fluorouridine,5-methyluridine and azauridine were synthesized from uridine by pUDP,the yield was 61.7%and 47.2%respectively.Deoxynucleosides were also synthesized by pUDP,but the yield was only about 20%.
Recombinant Escherichia coli pUDP, which overexpressed uridine phosphorylase (UPase), was constructed. 0.5 mmol.L-1 lactose had a similar induction effect as the commonly used inducer IPTG during 2.5-5.5 h of cell growth. The lactose-induced UPase was stable at 50 degrees C. Wet cells of pUDP was used as catalyst to biosynthesize 5-fluorouridine from 30 mmol.L-1 uridine and 5-fluorouracil in phosphate buffer (pH 7.0) catalyzed at 50 degrees C for 1.5 h and the yield of 5-fluorouridine was higher than 68%. Under the optimum reaction conditions for production of 5-fluorouridine, 5-methyluridine and azauridine were synthesized from uridine by pUDP, the yield was 61.7% and 47.2% respectively. Deoxynucleosides were also synthesized by pUDP, but the yield was only about 20%.