文献类型：期刊文献

中文题名：定向采收‘沪农灵芝1号’高三萜和水溶性多糖原料研究

英文题名：Directional Harvest of Ganoderma lucidum ‘Hunong No.1’ for High Triterpenoids and Water Soluble Polysaccharides

作者：马延梅[1];周帅[2];冯娜[2];刘艳芳[2];王金艳[2];唐庆九[2];韩伟[1];张劲松[2]

机构：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海200237;[2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发中心,上海市农业遗传育种重点实验室,上海201403

年份：2023

卷号：30

期号：1

起止页码：113

中文期刊名：食用菌学报

外文期刊名：Acta Edulis Fungi

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：上海市农业科学院卓越团队建设计划(沪农科卓2022-003)。

语种：中文

中文关键词：灵芝;三萜;多糖;栽培;超高效液相色谱-质谱;高效排阻色谱-多角度激光散射

外文关键词：Ganoderma lucidum;triterpenoid;polysaccharide;cultivation;UPLC-MS/MS;HPSECMALLS

摘要：采用超高效液相色谱-质谱(UPLC-MS/MS)法和高效排阻色谱-多角度激光散射(HPSEC-MALLS)法检测灵芝(Ganoderma lucidum)‘沪农灵芝1号’不同生长期(接种后2~7个月,分别命名为P1~P6)子实体和菌基中三萜和水溶性多糖含量,并结合子实体和菌基的产量计算三萜和水溶性多糖的产量。结果表明:子实体中灵芝酸C2、G含量在P1、P2期较高,灵芝烯酸B和灵芝酸D、F、DM含量在P2期最高,灵芝酸B、A、S、T和总三萜含量在P1期最高;菌基中灵芝酸C2、A、D、DM和灵芝烯酸B含量在P1期最高,灵芝酸G含量在P 5期最高,灵芝酸B、S含量在P 6期最高,灵芝酸F含量在P 1、P 6期较高,灵芝酸T、总三萜含量在P 5、P 6期较高。子实体中灵芝酸C2、G、B、D、F、DM,灵芝烯酸B,总三萜产量在P2期最高;灵芝酸A产量在P3期最高;灵芝酸S、T产量在P1期最高。菌基中灵芝酸C2、G、B、A、D、F、DM,灵芝烯酸B产量在P1期最高;灵芝酸S、T产量在P2期最高;总三萜产量在P1、P2期较高。子实体和菌基中的水溶性多糖含量和产量均在P1期最高。研究结果将为‘沪农灵芝1号’子实体的定向采收提供科学数据和指导。
In order to find the optimal harvest time for Ganoderma lucidum cultivar ‘Hunong No. 1’, ultrahigh performance liquid chromatography-mass spectrometry(UPLC-MS/MS) and high performance exclusion chromatography-multi angle laser scattering(HPSEC-MALLS) were used to determine contents of triterpenoids and water soluble polysaccharides in fruiting bodies and bases at different growth stages. From the second to the seventh month after inoculation, every month was defined as a growth stage, and the different growth stages were designated as P1 to P6, respectively. Based on the yields of fruiting bodies and bases, the yields of triterpenoids and water soluble polysaccharides at different growth stages were also calculated. The results showed that the contents of ganoderic acid C2 and ganoderic acid G in fruiting bodies were high at P1and P2;the contents of ganoderenic acid B, ganoderic acid D, ganoderic acid F, and ganoderic acid DM in fruiting bodies were the highest at P2;the contents of ganoderic acid B, ganoderic acid A, ganoderic acid S, ganoderic acid T and total triterpenoids in fruiting bodies were the highest at P1;the contents of ganoderic acid C2, ganoderic acid A, ganoderic acid D, ganoderic acid DM and ganoderenic acid B in bases were the highest at P1;the content of ganoderic acid G in bases was the highest at P5;the contents of ganoderic acid B and ganoderic acid S in bases were the highest at P6;the content of ganoderic acid F in bases was high at P1 and P6;the contents of ganoderic acid T and total triterpenoids were high at P5 and P6. For triterpenoid yield in fruiting bodies, ganoderic acid C 2, ganoderic acid G, ganoderic acid B, ganoderic acid D, ganoderic acid F, ganoderic acid DM, ganoderenic acid B and total triterpenoids were the highest at P2;ganoderic acid A was the highest at P3;both ganoderic acid S and ganoderic acid T were the highest at P 1. For triterpenoid yield in bases, ganoderic acid C 2, ganoderic acid G, ganoderic acid B, ganoderic acid A, ganoderic acid D, ganoderic acid F, ganoderic acid DM, ganoderenic acid B were the highest at P1;both ganoderic acid S and ganoderic acid T were the highest at P2;total triterpenoid yield was high at P1 and P2. For both fruiting body and base, the content and yield of water soluble polysaacharides were the highest at P1. These results provided a scientific basis for timely harvest of G. lucidum ‘Hunong No. 1’.