文献类型：期刊文献

中文题名：氯氟醚菊酯对斑马鱼胚胎及仔鱼心脏发育的毒性及氧化损伤作用

英文题名：Toxicity and oxidative damage of meperfluthrin on cardiac development ofzebrafish embryos and larvae

作者：李瑞瑞[1];周宁[1];吴梦琪[1];徐文平[1];陶黎明[1];张阳[1]

机构：[1]上海市化学生物学重点实验室,华东理工大学药学院,上海200237

年份：2024

卷号：26

期号：1

起止页码：149

中文期刊名：农药学学报

外文期刊名：Chinese Journal of Pesticide Science

收录：CSTPCD;;EI(收录号：20251918366766);北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家重点研发计划项目(2022YFD1700501)。

语种：中文

中文关键词：卫生杀虫剂;氯氟醚菊酯;环境风险;斑马鱼;发育毒性;氧化应激

外文关键词：hygienic pesticides;meperfluthrin;environmental risk;zebrafish;developmental toxicity;oxidative stress

摘要：氯氟醚菊酯是目前中国市场上销量最大的卫生用杀虫剂品种之一,其环境安全风险受到广泛关注。本研究以斑马鱼为试验模型,评价了氯氟醚菊酯急性暴露对斑马鱼胚胎及仔鱼心脏发育的毒性及氧化损伤作用。结果表明,氯氟醚菊酯对斑马鱼胚胎的急性毒性72 h-LC_(50)值为0.756μg/mL,属高毒。经氯氟醚菊酯不同剂量(0、0.2、0.4、0.8μg/mL)暴露72 h后发现:0.4和0.8μg/mL剂量处理组的仔鱼出现脊柱和尾部弯曲、心包和卵黄囊水肿等畸形特征,且心率和孵化率显著降低、胚胎发育不完整(体长变短、头部和眼睛面积缩小);0.2、0.4和0.8μg/m L的氯氟醚菊酯还能够诱导仔鱼心房心室分化,心脏功能相关基因myl7、myh6、vmhc的表达水平下调,并引起丙二醛(MDA)含量增加,以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)等抗氧化酶活性的降低,使仔鱼心脏处堆积活性氧(ROS)。本研究结果表明,氯氟醚菊酯暴露对斑马鱼胚胎的心脏发育具有显著毒性,且与氧化损伤作用相关。
Meperfluthrin is one of the top-selling sanitary insecticide species in the domestic market,drawing widespread attention due to its environmental safety risk.In this paper,zebrafish was used as an experimental model to assess the cardiac developmental toxicity and oxidative damage caused by acute exposure to meperfluthrin in embryos and larvae.The acute toxicity assessment revealed that meperfluthrin is highly toxic to zebrafish embryos with a 72 h-LC_(50) of 0.756μg/mL.After exposure to different doses of meperfluthrin(0,0.2,0.4,0.8μg/mL)for 72 h,the larvae from 0.4 and 0.8μg/mL dose-treated groups showed deformities,including spine and tail curvature,pericardium and yolk sac edema, significant reduction of heart rate and hatchability, and incomplete embryonic development(shorter body length, smaller head, and eye area). Moreover, meperfluthrin of 0.2, 0.4, and 0.8 μg/mLinduced atrial and ventricular differentiation in larvae, and down-regulated the expression of cardiacfunction-related genes myl7, myh6, and vmhc. It also increased the content of malondialdehyde (MDA),as well as decreased the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase(CAT), and glutathione peroxidase (GSH-Px), resulting in the accumulation of reactive oxygen species(ROS) in the heart of larvae. The results of this paper indicate that meperfluthrin exposure has significanttoxicity to the cardiac development of zebrafish embryos and is associated with oxidative damage.