文献类型：期刊文献

英文题名：A novel polydopamine-based chemiluminescence resonance energy transfer method for microRNA detection coupling duplex-specific nuclease-aided target recycling strategy

作者：Wang, Qian[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2016

卷号：80

起止页码：366

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20160701914404);WOS:【SCI-EXPANDED(收录号:WOS:000372558500052)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grant 21335003), the Key Grant Project of Chinese Ministry of Education (Grant 313019), and the Fundamental Research Funds for the Central Universities (Grants WF1514038 and WF1516011).

语种：英文

外文关键词：MicroRNA detection; Chemiluminescence resonance energy transfer; Polydopamine; Duplex-specific nuclease; Signal amplification

摘要：MicroRNAs (miRNAs), functioning as oncogenes or tumor suppressors, play significant regulatory roles in regulating gene expression and become as biomarkers for disease diagnostics and therapeutics. In this work, we have coupled a polydopamine (PDA) nanosphere-assisted chemiluminescence resonance energy transfer (CRET) platform and a duplex-specific nuclease (DSN)-assisted signal amplification strategy to develop a novel method for specific miRNA detection. With the assistance of hemin, luminol, and H2O2, the horseradish peroxidase (HRP)-mimicking G-rich sequence in the sensing probe produces chemiluminescence, which is quickly quenched by the CRET effect between PDA as energy acceptor and excited luminol as energy donor. The target miRNA triggers DSN to partially degrade the sensing probe in the DNA-miRNA heteroduplex to repeatedly release G-quadruplex formed by G-rich sequence from PDA for the production of chemiluminescence. The method allows quantitative detection of target miRNA in the range of 80 pM-50 nM with a detection limit of 49.6 pM. The method also shows excellent specificity to discriminate single-base differences, and can accurately quantify miRNA in biological samples, with good agreement with the result from a commercial miRNA detection kit. The procedure requires no organic dyes or labels, and is a simple and cost-effective method for miRNA detection for early clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.