文献类型：期刊文献

英文题名：One-step production of 2,3-butanediol from starch by secretory over-expression of amylase in Klebsiella pneumoniae

作者：Zheng, Yu[1]; Zhang, Haiyi[1]; Zhao, Li[1]; Wei, Liujing[1]; Ma, Xingyuan[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

年份：2008

卷号：83

期号：10

起止页码：1409

外文期刊名：Journal of Chemical Technology and Biotechnology

收录：EI(收录号：20084211637978)

语种：英文

外文关键词：Bacteria - Amylases - Gene expression - Substrates

摘要：Background: 2,3-Butanediol (2,3-BD) is a valuable chemical that can be biosynthesized from many kinds of substrates. For commercial biological production of 2,3-BD, it is desirable to use cheap substrate without pretreatment, such as starch. However, there have been few reports on the production of 2,3-BD directly from starch. Results: In this work, gene malS coding for α-amylase (EC 3.2.1.1) precursor was inserted into plasmid pUC18K, and secretory over-expression of α-amylase was achieved by engineered Klebsiella pneumoniae. The extracellular recombinant amylase accelerated the hydrolyzation of starch, and one-step production of 2,3-BD from starch was carried out by engineered K. pneumoniae. A 2,3-BD concentration of 3.8 g L-1 and yield of 0.19 g 2,3-BD g-1 starch were obtained after 24h fermentation. Conclusion: The one-step production of 2,3-BD from starch was achieved by secretory over-expression of amylase in K. pneumoniae. This would simplify the process and reduce the production cost considerably by enabling use of starch with minimal pretreatment. ? 2008 Society of Chemical Industry.