文献类型：期刊文献

英文题名：A dual-delivery system of pH-responsive chitosan-functionalized mesoporous silica nanoparticles bearing BMP-2 and dexamethasone for enhanced bone regeneration

作者：Gan, Qi[1,2,3];Zhu, Jiaoyang[1,2];Yuan, Yuan[1,2];Liu, Honglai[3];Qian, Jiangchao[1];Li, Yongsheng[2];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat Minist Educ, Key Lab Ultrafine Mat Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Chem Engn, Dept Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：3

期号：10

起止页码：2056

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20151000601974);WOS:【SCI-EXPANDED(收录号:WOS:000350689200003)】；

基金：The authors wish to express their gratitude to the financial supports from National Basic Research Program of China (973 Program, no. 2012CB933600), the 111 Project (B14018), National Natural Science Foundation of China (no. 31400817, no. 31470924 and no. 31330028), Chinese Postdoctoral Science Foundation (2013M541484). This study was also supported by Program for New Century Excellent Talents in University (no. NCET-11-0640) and the national Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204).

语种：英文

外文关键词：Bone - Targeted drug delivery - Sol-gels - Sol-gel process - Silica nanoparticles - Mesoporous materials - Proteins - Controlled drug delivery

摘要：Bone morphogenetic protein-2 (BMP-2) is considered one of the most effective and extensively used growth factors to induce osteoblast differentiation and accelerate bone regeneration. Dexamethasone (Dex) with suitable dosage can enhance BMP-2-induced osteoblast differentiation. To strengthen this synergistic osteoinductive effect, a pH-responsive chitosan-functionalized mesoporous silica nanoparticle (chi-MSN) ensemble was fabricated for dual-delivery of BMP-2 and Dex. The MSNs are prepared by a CTAB-templated sol-gel method, and further coated by chitosan via the crosslinking of glycidoxypropyltrimethoxysilane (GPTMS). The small Dex is encapsulated in the mesopores and the large BMP-2 is incorporated into the chitosan coating. These chi-MSNs can quickly release BMP-2 in a bioactive form and can then be efficiently endocytosed and further realize a controlled release of Dex with the decreased pH value into/in cells. With the synergistic action of BMP-2 and Dex outside and inside the cell, this dual hybrid delivery system can significantly stimulate osteoblast differentiation and bone regeneration in vitro and in vivo. Together, this dual-delivery strategy for osteogenic protein delivery may enhance clinical outcomes by retaining the bioactivity and optimizing the release mode of the drug/protein.