文献类型：期刊文献

中文题名：有机溶剂中逆胶束增溶醇脱氢酶的催化性能研究

英文题名：Catalytic Performance of Alcohol Dehydrogenase Solubilized by Reverse Micelles in Organic Solvent

作者：许建和[1];周洁[1];朱洁[1];刘洪来[1];胡英[1]

机构：[1]华东理工大学化学系

年份：1996

卷号：22

期号：3

起止页码：336

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

基金：国家自然科学基金

语种：中文

中文关键词：逆胶束;醇脱氢酶;有机溶剂;催化性能;增溶

外文关键词：reverse micelles;alcohol dehydrogenase;n-alkanols;oxidation reaction;organic solvents

摘要：考察了酵母醇脱氢酶在“水／气溶胶ＯＴ／有机溶剂”逆胶束系统中催化脂肪醇氧化反应的条件、性能及动力学。结果表明，在构成逆胶束连续相的各种有机溶剂中，疏水性越强则酶的活力越高，以异辛烷为最佳。逆胶束中酶反应的最适温度在２５℃左右；活化能约为５２ｋＪ／ｍｏｌ，比水相系统略低。逆胶束酶的活力随着系统的含水量Ｒ增加而升高，但应控制Ｒ＜５０，以不出现相变混浊为限。酶活力与ｐＨ的关系与底物醇的浓度有关，仅在浓度很低（［ｓ］＜Ｋｍ）时才呈现极值位于ｐＨ８．２的钟形曲线，一般在浓度较高时酶活力随ｐＨ增大而单调上升。酶在逆胶束中的底物特异性不同于普通的水相系统；正丁醇为最适底物，其表观米氏常数为３．３０ｍｍｏｌ／Ｌ，低于水溶液中的４．３４ｍｍｏｌ／Ｌ。
The catalytic conditions,performance and kinetics of Yeast Alcohol Dehydrogenase for the oxidation reactons of n-alkanols in reverse micellar'water/Aerosol OT/organic solvent'system were investigated.It was indicated that the more hydrophobic the organic solvent was,the higher activity the expresses,with isooctane being the best among various solvents examined.The optimum temperature for the enzyme in reverse micelles is around 25℃and the activation energy determined is about 52kJ/mol,which is a little lower than that reported in aqueous system.Activity of the enzyme in reverse micelles rises with the increase of water content R (H_2 O]/[AOT])until it reaches about 50 where phase transilation will occur.The high concentrations of substrate,far exceeding the Michaelis constant,cause the reaction rates to increase monotonically with the increase of pH, whereas small concentrations lower than give a maximum rate around pH 8.2.It was found that the substrate specificity of alcohol dehydrogenase in reverse micellar system was quite different from that in aqueous system and the apparent Km value for n-butanol,the most suitable substrate of the enzyme in reverse micelles,was 3.30mmol/L,which is smaller than that determined in bulk water,4.34mmol/L.