文献类型：期刊文献

中文题名：封闭式光生物反应器集胞藻6803光自养和混合营养培养比较

英文题名：Mixotrophic and Photoautotrophic Cultivation of Synechocystis sp. PCC 6803 in an Enclosed Photobioreactor

作者：王永红[1];李元广[1];施定基[2];沈国敏[1];茹炳根[3];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]中国科学院植物研究所光合作用研究中心,北京100093;[3]北京大学生命科学学院,北京100871

年份：2001

卷号：27

期号：3

起止页码：247

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金 !(2 98760 10 ) ;国家海洋生物技术 863!(819-Q-0 1) ;国家"九五"科技攻关 !(96-C0 2 -0 4-0 5 ) ;生物反

语种：中文

中文关键词：集胞藻6803;混合营养培养;光生物反应器;葡萄糖;蓝藻;基因工程;光自养培养

外文关键词：Synechocyatis sp. PCC 6803; mixotrophic; photobioreactor; glucose

摘要：采用封闭式光生物反应器进行了蓝藻基因工程常用宿主系统集胞藻 Synechocystis sp.PCC 680 3的混合营养培养 ,并与光自养培养进行了比较。在两种培养方式下 ,集胞藻 680 3的饱和光强基本相同 ,都为 50 0 0 lx。当入射光强为 50 0 0 lx、初始葡萄糖浓度为 1 .74g/L时 ,混合营养生长在葡萄糖消耗完 ( 69.5h)时的藻细胞密度为 1 .36g/L、叶绿素浓度为 2 0 .0 8mg/L、能量得率为1 6.7% ,分别为同期光自养生长的 3.8倍、2 .3倍和 2 .6倍。这表明封闭式光生物反应器混合营养培养方式在促进集胞藻 680
Compared with that of photoautotrophic cultivation, the characteristics of mixortophic cultivation of Synechocystis sp. PCC 6803 in an enclosed photobioreactor were investigated. The saturated level of light intensity of mixotrophic growth in the photobioreactor was 5 000 lx, which was the same as that of photoautotrophic growth. At incident light intensity 5 000 lx and initial glucose concentration 1.74g/L, after 69.5 h mixotrophic cultivation, 1.36g/L cell density, 20.08mg/L chlorophyll concentration and 16.7% energy efficiency were achieved, which were respectively 3.8 times, 2.3 times and 2.6 times as much as those in photoautotrophic cultivation. On the basis of above results, it was concluded that mixotrophic cultivation system had advantages over photoautotrophic cultivation system for Synechocystis sp. PCC 6803 on production of biomass, photosynthetic pigment and energy utilization.