文献类型：期刊文献

英文题名：Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis

作者：Chen, Fangping[1,2,4];Liang, Qing[2];Mao, Lijie[2];Yin, Yanrong[2];Zhang, Lixin[2];Li, Cuidi[3];Liu, Changsheng[1,2,4]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Shanghai Inst Traumatol & Orthopaed, Ruijin Hosp,Sch Med, Dept Orthopaed,Shanghai Key Lab Prevent & Trea, Shanghai 200025, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Mat & Dynam Chem, Shanghai 200237, Peoples R China

年份：2022

卷号：10

起止页码：335

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000743259000006)】；

基金：This investigation was funded by National Key Research and Development Program of China (No.2016YFC1102900), National Natural Science Foundation of China (No. 51772100 and No. 32171342), Shanghai Science and Technology Agriculture Project (No. 202002080002F01474), Shanghai Pujiang Program (16PJD015) and Joint Fund for equipment pre-research of the ministry of education (6141A02022618).

语种：英文

外文关键词：Asperosaponin VI; rhBMP-2; Osteoporosis; Osteogenesis; Osteoclastogenesis

摘要：Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function. Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis. In this study, we first systematically investigated the roles of Chinese medicine Asperosaponin VI (ASP VI) on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs, and then explored the synergistic effect of ASP VI and BS (BMP-2 immobilized in 2-N, 6-O-sulfated chitosan) on bone formation. The result showed that ASP VI with the concentration lower than 10(-4) M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs. Simultaneously, ASP VI significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL. Furthermore, by stimulating the SMADs, TGF-beta 1, VEGFA, and OPG/RANKL signaling pathways, ASBS (ASP VI and BS) substantially enhanced osteogenesis, greatly promoted angiogenesis, and suppressed osteoclastogenesis. The findings provide a new perspective on osteoporosis care and prevention.