文献类型：期刊文献

英文题名：A colorimetric and fluorescent dual probe for specific detection of cysteine based on intramolecular nucleophilic aromatic substitution

作者：Ma, Limin[1];Qian, Junhong[1];Tian, Haiyu[1];Lan, Minbo[1,2];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2012

卷号：137

期号：21

起止页码：5046

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000309427600023)】；

基金：This work was financially supported by the National 973 Program (no. 2011CB910404), NSFC (no. 21074033 and 21075039), Science and Technology Commission of Shanghai Municipality (STCSM no. 10dz2220500) and the Open Project Fund of Shanghai Key Laboratory of Scene Material Evidence (2011XCWZK02). Dr J. Qian thanks Prof. A. M. Brouwer (University of Amsterdam) and Prof. Y. Yang (East China University of Science & Technology) for their kind help.

语种：英文

摘要：4-Nitro-1,8-naphthalic anhydride (NNA) was used to distinguish cysteine from homocysteine and other potentially interfering thiols through a novel sequential substitution mechanism. The discrimination involves a blue-fluorescent thioether formation via nucleophilic aromatic substitution of the nitro group by thiol, followed by a second intramolecular nucleophilic aromatic substitution of alkylthio with the amino group to give the green-fluorescent 4-amino derivative. NNA is highly selective towards Cys, and the detection limit of Cys by this method is 0.3 mu M.