文献类型：期刊文献

英文题名：Analysis of small interfering RNA by capillary electrophoresis in hydroxyethylcellulose solutions

作者：Liu, Chenchen[1];Yamaguchi, Yoshinori[2,3];Zhu, Xifang[4];Li, Zhenqing[1];Ni, Yi[2];Dou, Xiaoming[2,4]

机构：[1]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, Shanghai, Peoples R China;[2]E China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed IPBM, Shanghai 200237, Peoples R China;[3]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Suita, Osaka 5650871, Japan;[4]Changzhou Inst Technol, Sch Optoelect Engn, Changzhou, Jiangsu, Peoples R China

年份：2015

卷号：36

期号：14

起止页码：1651

外文期刊名：ELECTROPHORESIS

收录：;EI(收录号：20231613910182);WOS:【SCI-EXPANDED(收录号:WOS:000358010400015)】；

基金：This project was partly supported by Grand-in-Aid for Scientific Research (Houga) (no. 25600049 [Y. Y.]), JSPS, Japan and The Innovation Fund Project for Graduate Student of Shanghai (no. JWCXSL1401), China.

语种：英文

外文关键词：Capillary electrophoresis; double-stranded RNA; double-stranded DNA; Hydroxyethylcellulose; small interfering RNA

摘要：The analysis of small interfering RNA (siRNA) is important for gene function studies and drug developments. We employed CE to study the separation of siRNA ladder marker, which were ten double-stranded RNA (dsRNA) fragments ranged from 20 to 1000 bp, in solutions of hydroxyethylcellulose (HEC) polymer with different concentrations and molecular weights (Mws). Migration mechanism of dsRNA during CE was studied by the mobility and resolution length (RL) plots. We found that the RL depended on not only the concentration of HEC, but also the Mw of HEC. For instance, RL of small dsRNA fragment was more influenced by concentration of high Mw HEC than large dsRNA fragment and RL of large dsRNA fragment was more influenced by concentration of low Mw HEC than small dsRNA fragment. In addition, we found electrophoretic evidence that the structure of dsRNA was more compact than dsDNA with the same length. In practice, we succeeded to separate the glyceraldehyde 3-phosphate dehydrogenase siRNA in the mixture of the siRNA ladder marker within 4 min.