文献类型：期刊文献

中文题名：近平滑假丝酵母细胞催化乙酰基三甲基硅烷不对称还原反应

英文题名：Asymmetric Reduction of Acetyltrimethylsilane Catalyzed by Candida parapsilosis CCTCC M203011 Cells

作者：娄文勇[1];郭强[1];郁惠蕾[2];宗敏华[1]

机构：[1]华南理工大学轻工与食品学院制浆造纸工程国家重点实验室,广东广州510640;[2]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2009

卷号：30

期号：12

起止页码：1276

中文期刊名：催化学报

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(20602013;20876059);华东理工大学生物反应器工程国家重点实验室开放课题基金

语种：中文

中文关键词：近平滑假丝酵母细胞;乙酰基三甲基硅烷;(R)-1-三甲基硅乙醇;不对称还原

外文关键词：Candida parapsilosis CCTCC M203011 cell; acetyltrimethylsilane; （R）-1-trimethylsilylethanol; asymmetric reduction;

摘要：采用近平滑假丝酵母细胞用于催化乙酰基三甲基硅烷不对称还原反应,可高选择性地生成(R)-1-三甲基硅乙醇.结果表明,固定化于海藻酸钙的细胞催化该反应的产物收率比游离细胞的高.不同辅底物对该反应的影响显著,以葡萄糖为辅底物时,反应的初速率较快,产物收率较高.该反应的最适条件为:辅底物(葡萄糖)浓度110mmol/L,振荡速度180r/min,缓冲液pH值6.0,反应温度30oC,底物浓度20mmol/L.在此反应条件下反应的初速率、产物收率和产物的ee值分别为11.4μmol/h,96.5%和99.9%.
The asymmetric reduction of acetyltrimethylsilane （ATMS） to （R）-1-trimethylsilylethanol （TMSE） catalyzed by Candida parapsilosis CCTCC M203011 cells was studied. It was found that the Candida parapsilosis CCTCC M203011 cells were capable of mediating the reduction of ATMS to （R）-1-TMSE with excellent enantioselectivity. The yield of （R）-1-TMSE with the cells immobilized on calcium alginate was higher than that with free cells. In addition, different co-substrates manifested a distinct effect on the bioreduction,and the initial reaction rate was clearly faster and the product yield was much higher using glucose as a co-substrate in the bioreduction. Moreover, the optimal glucose concentration, shaking rate, buffer pH, reaction temperature, and substrate concentration for the bioreduction were found to be 110 mmol/L, 180 r/min, 6.0, 30 ℃, and 20 mmol/L, respectively. Under these conditions, the initial reaction rate, maximum product yield, and product ee value were 11.4 mmol/h, 96.5%, and 99.9%, respectively.