文献类型：期刊文献

英文题名：Content determination of the flavonoids in the different parts and different species of Abelmoschus esculentus L. by reversed phase-high performance liquid chromatograph and colorimetric method

作者：Lin, Yin[1];Lu, Min-feng[2];Liao, Hai-bing[2];Li, Yu-xian[3];Han, Wei[1];Yuan, Ke[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Zhejiang Agr & Forestry Univ, Linan 311300, Zhejiang, Peoples R China;[3]Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450008, Henan, Peoples R China

年份：2014

卷号：10

期号：39

起止页码：278

外文期刊名：PHARMACOGNOSY MAGAZINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000340485200014)】；

基金：This project is sponsored by Zhejiang Provincial Natural Science Foundation of China (LY13H280011), Conflict of Interest: None declared.

语种：英文

外文关键词：Abelmoschus esculentus; flavonoid glycoside; reversed-phase high-performance liquid chromatograph; total flavonoids

摘要：Background: This research will establish the ultraviolet colorimetric method to determine the total flavonoid content in different species and different parts of Abelmoschus esculentus L. Materials and Methods: We establish the reversed-phase high-performance liquid chromatograph (RP-HPLC) method to determine the content of the three flavonoid glycosides in different species and different parts of the A. esculentus. Adopt the NaNO2-Al (NO3)(3)-NaOH colorimetric method to determine the total flavonoid content; at the same time, adopt the RP-HPLC method to determine the contents of the three flavonoid glycosides. Using the methods of ultraviolet colorimetry and RP-HPLC, we determined and analyzed the total flavonoid content and the content of the three flavonoid glycosides in different species and different parts of A. esculentus. Results: There are great distribution differences of the total flavonoids and the three flavonoid glycosides in different species and parts of A. esculentus. Among them, the content of the effective constituents in the flower is relatively high, next is in the fruit. In the different species of A. esculentus, the content of the flavonoids of finger relatively high. The HPLC method established in this research is simple and convenient and its results are accurate and reliable. In addition, it has a very good repeatability. Conclusion: The results provided the reference data for the medicinal use of A. esculentus and it can be used in quality analyzing of its effective constituents.